Sekine K, Watanabe-Sekine E, Toida T, Kasashima T, Kataoka T, Hashimoto Y
Bio-Chemical Research Laboratory, Morinaga Milk Industry Co., Ltd., Kanagawa Prefecture, Japan.
Immunopharmacol Immunotoxicol. 1994 Nov;16(4):589-609. doi: 10.3109/08923979409019741.
We examined the adjuvant activity of the Bifidobacterial Cell Wall preparation (WPG) for in vivo immune responses in mice. We studied three classical immune responses, which are thought to be T-cell mediated responses, to evaluate the adjuvant activity of WPG. The delayed type hypersensitivity (DTH) responses of sheep blood red cell (SRBC)-sensitized mice were significantly augmented by WPG, although the enhancement varied with the timing, route and dosage of injection. The adjuvant activity of WPG was also confirmed by using a glutaraldehyde treated- and Concanavalin A associated- tumor vaccine (G-Con A tumor vaccine) system. BALB/c mice sensitized with G-Con A tumor vaccine and WPG improved synergistically in survival time and cure rate compared with those given G-Con A vaccine alone. Spleen cells of Meth A tumor-bearing mice induced antitumor neutralizing activity with the growth of tumor but the activity declined and disappeared at the late stage of tumor growth (over 28 days after tumor transplantation). On the other hand, antitumor neutralizing immunity was prolonged for as long as 33 days in mice inoculated with Meth A tumor and WPG. The requirement of a T-cell subpopulation in the spleen cells of tumor plus WPG treated mice was confirmed using anti-Thy 1.2 antiserum + complement to deplete them. The adjuvant activities of the Bifidobacterial cell wall demonstrated by the in vivo immune responses predict that Bifidobacteria may play a role as an immunomodulator in human and animal intestines.
我们研究了双歧杆菌细胞壁制剂（WPG）对小鼠体内免疫反应的佐剂活性。我们研究了三种经典的免疫反应，这些反应被认为是T细胞介导的反应，以评估WPG的佐剂活性。WPG显著增强了绵羊红细胞（SRBC）致敏小鼠的迟发型超敏反应（DTH），尽管增强程度随注射时间、途径和剂量而有所不同。通过使用戊二醛处理并与伴刀豆球蛋白A结合的肿瘤疫苗（G-Con A肿瘤疫苗）系统，也证实了WPG的佐剂活性。与单独给予G-Con A疫苗的小鼠相比，用G-Con A肿瘤疫苗和WPG致敏的BALB/c小鼠在存活时间和治愈率方面有协同改善。携带Meth A肿瘤的小鼠的脾细胞随着肿瘤生长诱导出抗肿瘤中和活性，但在肿瘤生长后期（肿瘤移植后超过28天）该活性下降并消失。另一方面，在接种Meth A肿瘤和WPG的小鼠中，抗肿瘤中和免疫延长至长达33天。使用抗Thy 1.2抗血清加补体来耗尽肿瘤加WPG处理小鼠脾细胞中的T细胞亚群，证实了其对T细胞亚群的需求。双歧杆菌细胞壁的体内免疫反应所显示的佐剂活性预示着双歧杆菌可能在人和动物肠道中作为免疫调节剂发挥作用。
