Salvadori L G, Blake M S, McCarty M, Tai J Y, Zabriskie J B
Laboratory of Clinical Microbiology/Immunology, Rockefeller University, New York, New York 10021.
J Infect Dis. 1995 Mar;171(3):593-600. doi: 10.1093/infdis/171.3.593.
Antibodies reactive with group A streptococci (GAS) carbohydrate were studied by ELISA and in an indirect bactericidal assay. The ELISA used GAS carbohydrate covalently bound to phosphatidylethanolamine incorporated into liposomes so that both precipitating and nonprecipitating antibodies were measured. Sera from children from different geographic areas exhibited marked differences in levels of anti-GAS carbohydrate antibody, which increased with age. The antibodies were predominantly of IgG. In bactericidal assays, most of these sera promoted phagocytosis of several type-specific M-positive strains. Opsonization was also related to serum levels of anti-GAS carbohydrate antibodies. These opsonizing antibodies were depleted from the serum by absorption of the sera on an N-acetyl-D-glucosamine affinity column. Antibody eluted from this column could partially restore opsonization of GAS. Anti-GAS carbohydrate antibodies play a major role in these opsonophagocytosis assays.
通过酶联免疫吸附测定（ELISA）和间接杀菌试验研究了与A组链球菌（GAS）碳水化合物反应的抗体。ELISA使用与掺入脂质体的磷脂酰乙醇胺共价结合的GAS碳水化合物，以便同时检测沉淀抗体和非沉淀抗体。来自不同地理区域儿童的血清在抗GAS碳水化合物抗体水平上表现出显著差异，且该抗体水平随年龄增长而升高。这些抗体主要为IgG。在杀菌试验中，大多数此类血清促进了几种特定M型阳性菌株的吞噬作用。调理作用也与抗GAS碳水化合物抗体的血清水平相关。通过将血清吸附在N-乙酰-D-葡萄糖胺亲和柱上，可从血清中去除这些调理抗体。从该柱洗脱的抗体可部分恢复GAS的调理作用。抗GAS碳水化合物抗体在这些调理吞噬试验中起主要作用。
