爱荷华州母猪中的刚地弓形虫：通过小鼠和猫的生物测定法对刚地弓形虫分离物的抗体滴度比较
Toxoplasma gondii in Iowa sows: comparison of antibody titers to isolation of T. gondii by bioassays in mice and cats.
作者信息
Dubey J P, Thulliez P, Powell E C
机构信息
Parasite Biology and Epidemiology Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705-2350.
出版信息
J Parasitol. 1995 Feb;81(1):48-53.
Hearts of 1,000 pigs killed at an abattoir in Iowa were bioassayed for the prevalence of tissue cysts of Toxoplasma gondii. One hundred grams of cardiac muscle from each pig was homogenized, digested in pepsin solution, and bioassayed in 10 mice. Five hundred grams of heart tissue from each of a subset of 183 pigs was also bioassayed in cats. Serum collected from the heart from each pig was assayed for anti-T. gondii antibodies in the modified agglutination test using formalin-fixed whole tachyzoites. Anti-T. gondii antibodies were found in 22.2% of pigs. Viable T. gondii was isolated from a total of 170 pigs; from 50 hearts by bioassay in mice, from 58 hearts by bioassay in both mice and cats, and from 62 pigs by bioassay in cats only. The success of isolation in cats (65.6%) was approximately twice that in mice (31.7%). Percentage of isolations of T. gondii with respect to reciprocal antibody titers (in parentheses) in pigs was: 3.7% (< 20), 37.1% (20), 38.1% (40), 60% (80), 75% (200), 77% (400), 83% (800), and 75.8% (> or = 2,000 to 16,000).
对在爱荷华州一家屠宰场宰杀的1000头猪的心脏进行生物测定，以检测弓形虫组织囊肿的流行情况。从每头猪身上取100克心肌进行匀浆，在胃蛋白酶溶液中消化，并在10只小鼠身上进行生物测定。还从183头猪的一个子集中的每头猪身上取500克心脏组织在猫身上进行生物测定。从每头猪的心脏采集血清，使用福尔马林固定的全速殖子在改良凝集试验中检测抗弓形虫抗体。在22.2%的猪中发现了抗弓形虫抗体。总共从170头猪中分离出了活的弓形虫；通过在小鼠身上进行生物测定从50个心脏中分离出，通过在小鼠和猫身上进行生物测定从58个心脏中分离出，仅通过在猫身上进行生物测定从62头猪中分离出。在猫身上分离成功的比例（65.6%）约为在小鼠身上的两倍（31.7%）。猪中弓形虫分离率相对于抗体效价倒数（括号内）的百分比为：3.7%（<20），37.1%（20），38.1%（40），60%（80），75%（200），77%（400），83%（800），以及75.8%（≥2000至16000）。
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