Cobb C M, Helber J T, Hirschberg R
Department of Periodontics, School of Dentistry, University of Missouri-Kansas City 64108.
J Periodontal Res. 1994 Nov;29(6):410-7. doi: 10.1111/j.1600-0765.1994.tb01242.x.
Eikenella corrodens is a gram-negative, human pathogen which exhibits colony morphology variation. Scanning electron microscopy (SEM) was used to examine large (non-corroding) and small (corroding) colony variants from the type strain (ATCC 23834) and two clinical isolates (strains VA1 and CM1). Large colonies were large, flatter, and appeared relatively featureless compared to small colonies and had even, smooth colony margins. Small colonies were more raised from the medium surface, and often had a central raised region surrounded by flatter border. Cells on the surface of large colonies were more regularly arranged at the colony edge, and end-to-end rows of cells around the colony were seen in some strains. Cells in the center of the upper surface of small colonies were usually randomly arranged. Within cross sections of small colonies, cells were arranged randomly or perpendicular to the medium; in large colonies, cells were random or arranged horizontally. Amorphous, "slime" material was often seen covering groups of cells in large and small variants. An unusual variant, possibly a mutant, which combined features of both colony types was isolated.
腐蚀埃肯菌是一种革兰氏阴性人类病原体，其菌落形态会发生变化。使用扫描电子显微镜（SEM）检查了来自模式菌株（ATCC 23834）以及两个临床分离株（VA1和CM1菌株）的大菌落（不腐蚀型）和小菌落（腐蚀型）变体。与小菌落相比，大菌落更大、更扁平，外观相对无特征，且菌落边缘均匀、光滑。小菌落从培养基表面隆起更高，通常有一个中央隆起区域，周围是较扁平的边缘。大菌落表面的细胞在菌落边缘排列更规则，在一些菌株中可见菌落周围细胞呈端对端排列。小菌落上表面中心的细胞通常随机排列。在小菌落的横截面中，细胞随机排列或垂直于培养基排列；在大菌落中，细胞随机排列或水平排列。在大、小变体中，常可见无定形的“黏液”物质覆盖细胞群。分离出了一种不寻常的变体，可能是突变体，它兼具两种菌落类型的特征。
