Sritharan V, Iralu J V, Barker R H
Center for Biotechnology, Anna University, Madras.
Mol Cell Probes. 1994 Oct;8(5):409-16. doi: 10.1006/mcpr.1994.1058.
We describe experiments comparing use of different DNA probes to detect mycobacteria in clinical specimens after PCR. The objective was to assess correlation between results using Mycobacterium genus-specific, and species-specific M. tuberculosis probes. Given sufficient concordance, sequential use of such probes would provide a useful screening tool. An evaluation of genus-specific probes compared use of repetitive sequences in the clone pMAv17 with the 65-kDa sequences. Sensitivity was 100% for pMAv17, 93% using the 65-kDa sequence; specificity was 70% for both. We then compared M. tuberculosis-specific probes developed by us (Tb400) with IS6110 and mpt40. Sensitivity using Tb400 was 100%; using IS6110 was 97%, and using mpt40 was 50%. Specificity using Tb400 and IS6110 was 68%, and was 70% using mpt40. Fourteen specimens which were PCR-positive and culture-negative, were positive using both genus probes, and the M. tuberculosis-specific probes Tb400, and IS6110. Ten of these were positive using mpt40.
我们描述了一些实验，这些实验比较了在PCR之后使用不同DNA探针检测临床标本中分枝杆菌的情况。目的是评估使用分枝杆菌属特异性探针和结核分枝杆菌种特异性探针所得结果之间的相关性。如果有足够的一致性，依次使用这些探针将提供一种有用的筛查工具。对属特异性探针的评估比较了克隆pMAv17中的重复序列与65-kDa序列的使用情况。pMAv17的灵敏度为100%，使用65-kDa序列时为93%；两者的特异性均为70%。然后我们将我们开发的结核分枝杆菌特异性探针（Tb400）与IS6110和mpt40进行了比较。使用Tb400的灵敏度为100%；使用IS6110为97%，使用mpt40为50%。使用Tb400和IS6110的特异性为68%，使用mpt40为70%。14份PCR阳性但培养阴性的标本，使用两种属探针以及结核分枝杆菌特异性探针Tb400和IS6110时均为阳性。其中10份使用mpt40时为阳性。
