Dittmar M T, Cichutek K, Fultz P N, Kurth R
Department of Medical Biotechnology, Paul-Ehrlich-Institut, Langen, Germany.
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1362-6. doi: 10.1073/pnas.92.5.1362.
Infection with the acutely pathogenic molecular virus clone SIVsmmPBj1.9, cloned from isolate PBj14 of simian immunodeficiency virus (SIV) from sooty mangabey monkeys (Cercocebus atys), leads to acute viral and often lethal disease within days or weeks. SIVsmmPBj1.9 has the unique property of replicating in nonstimulated peripheral blood mononuclear cells from pig-tailed macaques. In contrast, molecular virus clone SIVagm3mc of SIV from African green monkeys (Cercopithecus aethiops), which is apathogenic in its natural host and in pig-tailed macaques, is unable to grow in nonstimulated peripheral blood cells. Chimeric proviruses were constructed by exchanging defined regions of SIVagm3mc against comparable regions of SIVsmmPBj1.9. Four of five hybrid viruses generated by transfection into the CD4-positive T-cell line C8166 replicated in T-cell lines permissive for SIVagm3mc replication and in stimulated peripheral blood cells from pig-tailed macaques and from African green monkeys. Three hybrid viruses displayed the distinct biological property of SIVsmmPBj14 to replicate in nonstimulated peripheral blood cells from pig-tailed macaques and from African green monkeys. Replication in nonstimulated peripheral blood cells was dependent on the presence of the U3 promoter region of SIVsmmPBj1.9 within the viral long terminal repeat.
从煤烟猕猴（Cercocebus atys）的猿猴免疫缺陷病毒（SIV）分离株PBj14克隆而来的急性致病分子病毒克隆SIVsmmPBj1.9感染后，会在数天或数周内引发急性病毒感染，且通常是致命疾病。SIVsmmPBj1.9具有在未受刺激的猪尾猕猴外周血单核细胞中复制的独特特性。相比之下，来自非洲绿猴（Cercopithecus aethiops）的SIV分子病毒克隆SIVagm3mc在其自然宿主和猪尾猕猴中无致病性，且无法在未受刺激的外周血细胞中生长。通过将SIVagm3mc的特定区域与SIVsmmPBj1.9的可比区域进行交换构建了嵌合前病毒。转染到CD4阳性T细胞系C8166中产生的五种杂交病毒中有四种在允许SIVagm3mc复制的T细胞系以及来自猪尾猕猴和非洲绿猴的受刺激外周血细胞中复制。三种杂交病毒表现出SIVsmmPBj14在来自猪尾猕猴和非洲绿猴的未受刺激外周血细胞中复制的独特生物学特性。在未受刺激外周血细胞中的复制取决于病毒长末端重复序列中SIVsmmPBj1.9的U3启动子区域的存在。
