Corres González J M, Rodríguez Hernández C, Zea Mendoza A C, Sequí Navarro J, Sanchidrián Fernández I
Servicio de Reumatología, Hospital Ramón y Cajal, Madrid.
Rev Clin Esp. 1995 Jan;195(1):16-21.
To compare indirect immunofluorescence (IIF) with immunoblotting (IB) in the detection of antiribosomal antibodies (anti-P Ab) in patients with systemic lupus erythematosus (SLE) and to investigate the possible association between anti-P Ab with serological and clinical findings in SLE, particularly with neurological manifestations.
Serum specimens from 44 SLE patients and 10 healthy subjects were investigated for anti-P Ab using IB and IIF in rat triple substrate and HEp-2 cells. In SLE patients measurements were made of antinuclear Ab, anti-DNA ds Ab, anti-Sm Ab, anti-U1RNP Ab, anti-Ro Ab, and anti-La Ab. Clinical manifestations of SLE were collected retrospectively when the serological investigation was made.
Of the 44 serum specimens tested, 9 showed a ribosomal pattern with triple rat substrate; 8 of them were IB positive (sensitivity 88%; specificity 97%); 12 serum specimens showed a ribosomal pattern with HEp-2 cells by the IIF technique, 9 were positive by IB (sensitivity 100%; specificity 91%). All ten healthy subjects were negative both with IIF and with IB. The nine patients with anti-P Ab in IB (20.45%) had anti-Ro Ab (55% vs. 37%), Anti-Sm Ab (33% vs. 22%, and U1RNP Ab (33% vs. 20%) more frequently than the 35 negative cases. Central nervous system disease (33 vs. 14%), and particularly seizures (33% vs. 5%) and psychosis (22% vs. 8%) were more common in cases with anti-P Ab, but as with serological associations, none of them reached a statistical signification.
IIF with both rat triple substrate and HEp-2 cells is useful for the presumptive diagnosis of anti-P Ab in patients diagnosed with SLE. No significant serological or clinical association was found in patients with anti-P Ab, although neurological disease was more common in these cases.
比较间接免疫荧光法（IIF）和免疫印迹法（IB）检测系统性红斑狼疮（SLE）患者抗核糖体抗体（抗P抗体）的效果，并研究抗P抗体与SLE患者血清学及临床结果，尤其是与神经学表现之间的可能关联。
采用IB法和IIF法，以大鼠三重底物和人喉癌上皮细胞（HEp-2细胞）检测44例SLE患者和10例健康对照者血清中的抗P抗体。检测SLE患者的抗核抗体、抗双链DNA抗体、抗Sm抗体、抗U1核糖核蛋白抗体、抗Ro抗体和抗La抗体。回顾性收集血清学检测时SLE患者的临床表现。
在检测的44份血清标本中，9份在大鼠三重底物上显示核糖体模式；其中8份IB法阳性（敏感性88%；特异性97%）；12份血清标本经IIF技术在HEp-2细胞上显示核糖体模式，9份IB法阳性（敏感性100%；特异性91%）。10例健康对照者IIF法和IB法均为阴性。9例IB法检测抗P抗体阳性的患者（20.45%）抗Ro抗体（55%比37%）、抗Sm抗体（33%比22%）和抗U1核糖核蛋白抗体（33%比20%）阳性率高于35例阴性患者。中枢神经系统疾病（33%比14%），尤其是癫痫发作（33%比5%）和精神病（22%比8%）在抗P抗体阳性患者中更常见，但与血清学关联一样，均未达到统计学意义。
大鼠三重底物和HEp-2细胞的IIF法有助于SLE患者抗P抗体的初步诊断。抗P抗体阳性患者未发现明显的血清学或临床关联，尽管这些患者神经疾病更常见。
