Thibaut M, Ansel M
Parassitologia. 1975 Jan-Dec;17(1-3):49-56.
The combination of electron beam scanning and X-ray detection allows to use the scanning electron probe microanalyzer as a sensitive microscope to elemental constitution. This made it possible to investigate three species of the Aspergillus nidulans group: A. nidulans, A. unguis, A. variecolor. Qualitative differences were found between the three species. The application of microanalysis is regarded as a useful addition to more conventional methods used in histochemical studies. The advantages of examining specimens which have not been submitted to fixation, dehydration and embedding are briefly discussed.
电子束扫描与X射线检测相结合,使得扫描电子探针微分析仪能够作为一种对元素组成敏感的显微镜使用。这使得研究构巢曲霉组的三个物种成为可能:构巢曲霉、爪曲霉、杂色曲霉。发现这三个物种之间存在定性差异。微分析的应用被认为是对组织化学研究中使用的更传统方法的有益补充。文中简要讨论了检查未经固定、脱水和包埋的标本的优点。