Takeda M, Tang R, Shapiro E, Burnett A L, Lepor H
Department of Urology, New York University School of Medicine, New York.
Urology. 1995 Mar;45(3):440-6. doi: 10.1016/S0090-4295(99)80013-2.
To determine whether nitric oxide (NO) is a mediator of prostatic smooth muscle activity.
Pharmacologic experiments using electrical field stimulation (EFS) were performed on strips of human and canine prostate.
EFS alone elicited frequency-dependent contractions in preparations of human and canine prostates. The greatest contractile activity was achieved at 30 Hz. In the presence of 10(-5) M guanethidine (GUA) and 2 x 10(-6) M atropine (ATR), EFS elicited relaxation of canine prostate strips relative to baseline tension. A weak biphasic response consisting of initial relaxation and subsequent contraction relative to baseline tension was observed in the human prostate strips exposed to similar conditions. The smooth muscle activity observed in the presence of GUA plus ATR was attributed to nonadrenergic, noncholinergic (NANC) nerve transmission. 10(-4) M L-NG-nitroarginine methylester (NAME) significantly increased EFS-elicited NANC smooth muscle activity both in human and canine prostates. L-arginine, 10(-2) M, reversed the effect of L-NAME in human and canine prostates. Sodium nitroprusside, 10(-4) M, a donor of NO, caused relaxation of both human and canine prostates. The mean magnitude of the relaxant response/cross-sectional area in human prostate (2.64 +/- 0.4 g/cm2) was significantly greater than in the canine prostate (1.09 +/- 0.17 g/cm2) (P < 0.005).
These results provide compelling evidence that NO plays a role in mediating contractile function of human and canine prostates.
确定一氧化氮（NO）是否为前列腺平滑肌活动的介质。
对人及犬前列腺组织条进行使用电场刺激（EFS）的药理学实验。
单独的EFS可引起人及犬前列腺组织条频率依赖性收缩。在30Hz时收缩活性最强。在存在10⁻⁵M胍乙啶（GUA）和2×10⁻⁶M阿托品（ATR）的情况下，EFS使犬前列腺组织条相对于基线张力出现松弛。在暴露于类似条件的人前列腺组织条中观察到相对于基线张力的微弱双相反应，包括初始松弛和随后的收缩。在存在GUA加ATR的情况下观察到的平滑肌活动归因于非肾上腺素能、非胆碱能（NANC）神经传递。10⁻⁴M L-NG-硝基精氨酸甲酯（NAME）显著增加了人及犬前列腺中EFS诱导的NANC平滑肌活动。10⁻²M的L-精氨酸可逆转L-NAME在人及犬前列腺中的作用。10⁻⁴M硝普钠，一种NO供体，可引起人及犬前列腺的松弛。人前列腺中松弛反应/横截面积的平均幅度（2.64±0.4g/cm²）显著大于犬前列腺（1.09±0.17g/cm²）（P<0.005）。
这些结果提供了令人信服的证据，表明NO在介导人及犬前列腺的收缩功能中起作用。
