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[产青链霉菌启动子活性DNA片段的亚克隆与测序]

[Subcloning and sequencing of promoter active DNA fragment from Streptomyces lividans].

作者信息

Jiang H, Dong K, Huan L

机构信息

Institute of Microbiology, Academia Sinica, Beijing.

出版信息

Wei Sheng Wu Xue Bao. 1994 Dec;34(6):480-2.

PMID:7879388
Abstract

Random subfragments with strong promoter activities were isolated from 2. 1 kb fragment of pMG50-25 using a promoter-probe vector pIJ4083. One of the promoter-active region was narrowed down to a 220bp sequence. Putative promoter regions, SD sequence and start codons were found.

摘要

利用启动子探针载体pIJ4083从pMG50 - 25的2.1 kb片段中分离出具有强启动子活性的随机亚片段。其中一个启动子活性区域被缩小到一个220 bp的序列。发现了推定的启动子区域、SD序列和起始密码子。

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