Chen Y, Xue Y G, Dong K N, Liu M H
Institute of Microbiology, Academia Sinica Beijing.
Yi Chuan Xue Bao. 1991;18(4):370-7.
A 410-bp DNA fragment of Streptomyces griseus No.45 with in vivo promoter activity in E. coli has been isolated by subcloning the cloning strain E. coli No.8-1. The result of sequencing shows that the promoter fragment contains 50.5% G + C bases. It has some repeat sequences. It shows good homology to the E. coli promoter consensus sequences in -10 and -35 regions. There are 18 bp between the two regions. It has two regions that are similar to SD sequence in E. coli and consensus sequence in SEP (Streptomyces-E.coli-type promoter) of Streptomyces lividans respectively. There is 1 Alu I site, 1 Cla I site, 2 Mbo I sites, 3 NlaIII sites and 1 PvuII site in the promoter fragment.
通过对克隆菌株大肠杆菌No.8-1进行亚克隆，分离出了灰色链霉菌No.45的一段410 bp的DNA片段，该片段在大肠杆菌中具有体内启动子活性。测序结果表明，该启动子片段含有50.5%的G + C碱基。它有一些重复序列。在-10和-35区域与大肠杆菌启动子共有序列具有良好的同源性。两个区域之间有18 bp。它有两个区域分别类似于大肠杆菌中的SD序列和淡紫链霉菌SEP（链霉菌-大肠杆菌型启动子）中的共有序列。该启动子片段中有1个Alu I位点、1个Cla I位点、2个Mbo I位点、3个NlaIII位点和1个PvuII位点。
