Levina L A, Zaĭtseva E V, Temper R M, Chulok T A
Zh Mikrobiol Epidemiol Immunobiol. 1994 Sep-Oct(5):63-6.
The toxicity, immunogenic properties and protective activity of the live culture of E. coli M17 and antigenic preparations obtained from cell suspensions of this strain have been studied under experimental conditions. As revealed in experiments on mice, E. coli M17 live culture has low virulence, moderate toxicity and provides the protection of immunized mice from challenge with homologous and highly virulent E. coli strains. E. coli M17 live culture, when introduced orally or intravenously into rabbits, ensures the synthesis of 02 and H6 antibodies. Blood sera taken from immunized rabbits yield better results than initial sera in experiments on the passive protection of mice. The results of our experiments show the expediency of the clinical trials of Colibacterin as a perspective Escherichia live oral vaccine.
在实验条件下，对大肠杆菌M17活培养物以及从该菌株细胞悬液中获得的抗原制剂的毒性、免疫原性和保护活性进行了研究。在小鼠实验中发现，大肠杆菌M17活培养物毒力低、毒性中等，可保护免疫小鼠免受同源和高毒力大肠杆菌菌株的攻击。将大肠杆菌M17活培养物经口或静脉注射给兔子后，可确保02和H6抗体的合成。在小鼠被动保护实验中，免疫兔子的血清比初始血清效果更好。我们的实验结果表明，将大肠杆菌菌苗作为一种有前景的大肠杆菌口服活疫苗进行临床试验是可行的。
