Scippo M L, Degand G, Duyckaerts A, Maghuin-Rogister G, Delahaut P
Laboratoire d'Analyse des Denrées Alimentaires d'Origine Animale, Faculté de Médecine Vétérinaire, Université de Liège, Belgium.
Analyst. 1994 Dec;119(12):2639-44. doi: 10.1039/an9941902639.
In the context of the control of the illegal administration of natural steroid hormones in cattle husbandry, an attempt was made to establish the decision levels for sex steroid hormones in the plasma of adult cattle, taking into account the effect of the treatment. Bulls and heifers were treated with two injections, at a two week interval, of an estradiol-testosterone cocktail. Steroid hormone and biochemical precursor concentrations were measured in plasma samples by using specific radioimmunoassays, before and after the treatment. When the treatment significantly (p < 0.05) modified a hormone concentration, a decision level was established for that hormone concentration. At each decision level, a score was assigned that represented the percentage of treated animals detected when the decision limit was applied. For heifers, 17 beta-estradiol and testosterone concentrations in plasma, which increased after the treatment, are the best criteria to use to detect treated animals, with decision limits of 20 pg ml-1 and 125 pg ml-1, respectively. In the instance of bulls, both testosterone and steroid biochemical precursor concentrations decreased in the plasma after the treatment. We proposed decision limits of 1500 pg ml-1 and 28 pg ml-1 for testosterone and androstenedione concentrations, respectively, the bulls displaying concentrations below these limits being positive. We observed that the repetition of the injection increased the score of the decision limit. The scores for testosterone are 70%, 14d after the first injection and 100% 14 d after the second injection, and for androstenedione, these scores are 60 and 100%, respectively.
在控制畜牧业中非法使用天然甾体激素的背景下，考虑到治疗效果，试图确定成年牛血浆中性甾体激素的判定水平。公牛和小母牛每隔两周接受两次雌二醇 - 睾酮混合物注射。在治疗前后，通过使用特定的放射免疫测定法测量血浆样本中的甾体激素和生化前体浓度。当治疗显著（p < 0.05）改变激素浓度时，为该激素浓度确定一个判定水平。在每个判定水平上，赋予一个分数，该分数代表应用判定限值时检测到的经治疗动物的百分比。对于小母牛，治疗后血浆中17β - 雌二醇和睾酮浓度升高，是检测经治疗动物的最佳标准，判定限值分别为20 pg/ml和125 pg/ml。在公牛的情况下，治疗后血浆中睾酮和甾体生化前体浓度均下降。我们分别提出睾酮和雄烯二酮浓度的判定限值为1500 pg/ml和28 pg/ml，血浆中浓度低于这些限值的公牛为阳性。我们观察到重复注射会提高判定限值的分数。睾酮的分数在第一次注射后14天为70%，第二次注射后14天为100%，而雄烯二酮的分数分别为60%和100%。
