毛发中盐酸克伦特罗残留量的检测

Detection of clenbuterol residues in hair.

作者信息

Adam A, Gervais N, Panoyan A, Ong H, Beliveau L, Ayotte C, Delahaut P

机构信息

Faculté de Pharmacie, Université de Montréal, Québec, Canada.

出版信息

Analyst. 1994 Dec;119(12):2663-6. doi: 10.1039/an9941902663.

DOI:10.1039/an9941902663

PMID:7879871

Abstract

Sixty rats were grown in the presence of 10 (n = 30) and 100 (n = 30) micrograms kg-1 body mass of clenbuterol for a period of 10 d. An immunoextraction step coupled with a competitive enzyme immunoassay allowed the quantification of clenbuterol in hair upon 20 (10 micrograms kg-1) and 30 d (10 micrograms kg-1) after the last dose. This accumulation in hair contrasts with the rapid clearance in tissues. The nature of the immunoreactive material was confirmed by mass spectrometry.

摘要

六十只大鼠在每千克体重分别为10微克（n = 30）和100微克（n = 30）的克伦特罗环境中饲养10天。免疫提取步骤结合竞争性酶免疫测定法能够在末次给药后20天（每千克体重10微克组）和30天（每千克体重10微克组）对毛发中的克伦特罗进行定量。毛发中的这种蓄积与组织中的快速清除形成对比。通过质谱法确认了免疫反应性物质的性质。