Elliott C T, McCaughey W J, Shortt H D
Food Addit Contam. 1993 Mar-Apr;10(2):231-44. doi: 10.1080/02652039309374145.
Reports of the illegal use of clenbuterol as a growth promotant prompted the development of a competitive enzyme immunoassay for this drug. This procedure was utilized to study the elimination of clenbuterol from tissues in sheep medicated with both therapeutic and growth-promoting doses of the drug. The results indicated that prior to removal of medication clenbuterol was widely distributed throughout the animal tissues. However as the withdrawal periods increased fluid targets such as urine and bile became less effective at detecting clenbuterol usage. At both therapeutic and growth-enhancing concentrations of clenbuterol liver samples remained positive up to the maximum withdrawal time given in this experiment (15 days). Concentrations of clenbuterol likely to cause food poisoning (> 100 ng/g) were only detected in liver samples taken prior to the removal of medication. The highest recorded concentration of clenbuterol in muscle was 22.5 ng/g.
非法使用盐酸克伦特罗作为生长促进剂的报道促使人们开发了一种针对该药物的竞争性酶免疫测定法。该方法被用于研究在给予治疗剂量和促生长剂量药物的绵羊体内，盐酸克伦特罗在组织中的消除情况。结果表明，在停药前，盐酸克伦特罗广泛分布于动物组织中。然而，随着停药期的延长，尿液和胆汁等液体样本在检测盐酸克伦特罗使用情况方面的效果变得较差。在盐酸克伦特罗的治疗浓度和促生长浓度下，肝脏样本在本实验给出的最长停药时间（15天）内均保持阳性。仅在停药前采集的肝脏样本中检测到可能导致食物中毒的盐酸克伦特罗浓度（>100纳克/克）。肌肉中记录到的盐酸克伦特罗最高浓度为22.5纳克/克。
