Salvadori M C, Rieser E M, Ribeiro Neto L M, Nascimento E S
Antidoping Lab-Jockey Club de São Paulo, Brazil.
Analyst. 1994 Dec;119(12):2701-3. doi: 10.1039/an9941902701.
The seeds of Guaraná are rich in xanthines and are used for the preparation of guaraná powder which is very commonly given to horses as a 'tonic' in Brazil. In this paper, the xanthine content of guaraná powder was determined, in addition to its clearance time in horses. Thin-layer chromatography was used as a screening procedure and high-performance liquid chromatography was performed to quantify the drugs in both the powder and urine samples. The guaraná powder was found to contain 2.16, 1.10 and 36.78 mg g-1 of theobromine (TB), theophylline (TP) and caffeine (CF), respectively, and in urine it was possible to detect TB and TP up to 13 d and CF up to 9 d after the administration of guaraná powder.
瓜拉那种子富含黄嘌呤，可用于制备瓜拉那粉，在巴西，瓜拉那粉常作为“滋补品”喂给马。本文测定了瓜拉那粉中的黄嘌呤含量及其在马体内的清除时间。采用薄层色谱法作为筛选程序，并进行高效液相色谱法对粉末和尿液样本中的药物进行定量分析。结果发现，瓜拉那粉中可可碱（TB）、茶碱（TP）和咖啡因（CF）的含量分别为2.16、1.10和36.78 mg g-1，在给马投喂瓜拉那粉后，在尿液中可检测到TB和TP长达13天，CF长达9天。
