Panteghini M, Bonora R, Pagani F
1st laboratory of Clinical Chemistry, Section of Clinical Chemistry and Enzymology, Spedali Civili, Brescia, Italy.
Ann Clin Biochem. 1994 Nov;31 ( Pt 6):544-9. doi: 10.1177/000456329403100603.
We have developed and optimized a procedure for the quantitation of non-enzymatically glycated apolipoprotein B (apo B). Glycated and non-glycated apo B were separated from serum using m-aminophenylboronate affinity chromatography, determined by immunophelometry and the percentage of glycated apo B was calculated. The measuring range of the assay was 2.9-185 mg/dL apo B. The within- and between-run coefficients of variation were < 7.4% and 14.6%, respectively, and recovery was > 98%. Free glucose in serum did not affect the results at concentrations below 25 mmol/L. In 45 non-diabetic subjects the mean concentration of glycated apo B was 4.3% (SD 1%). In type 1 (n = 17) and Type 2 (n = 60) diabetic patients the mean glycated apo B concentrations were 5.3% (SD 0.7%) and 5.9% (SD 1.1%), respectively, significantly higher than in controls (P < 0.001).
我们已经开发并优化了一种非酶糖基化载脂蛋白B（apo B）定量检测方法。采用间氨基苯硼酸亲和色谱法从血清中分离糖基化和非糖基化的apo B，通过免疫比浊法进行测定，并计算糖基化apo B的百分比。该检测方法的测量范围为2.9 - 185 mg/dL apo B。批内和批间变异系数分别< 7.4%和14.6%，回收率> 98%。血清中游离葡萄糖浓度低于25 mmol/L时不影响检测结果。45名非糖尿病受试者糖基化apo B的平均浓度为4.3%（标准差1%）。1型糖尿病患者（n = 17）和2型糖尿病患者（n = 60）糖基化apo B的平均浓度分别为5.3%（标准差0.7%）和5.9%（标准差1.1%），显著高于对照组（P < 0.001）。
