Panteghini M, Cimino A, Pagani F, Girelli A
1. Laboratorio Analisi Chimico-Cliniche, Spedali Civili, Brescia, Italy.
Clin Biochem. 1995 Dec;28(6):587-92. doi: 10.1016/0009-9120(95)00041-5.
To evaluate the level of nonenzymatic glycation of apolipoprotein B in patients with insulin and noninsulin dependent diabetes mellitus.
Using a method based on a combination of affinity chromatography and immunonephelometry, we measured the concentration of glycated apolipoprotein B (apo B) in serum of 140 diabetic patients, 43 insulin-dependent (IDDM), and 97 noninsulin-dependent (NIDDM), and 45 nondiabetic control subjects.
Although total apo B concentration in serum was significantly increased only in NIDDM patients, both groups of diabetics showed higher percentages of glycated apo B (IDDM, 4.84 +/- 0.8%; NIDDM, 5.61 +/- 1.1%) than did control subjects (4.28 +/- 1.0%), the greatest percentage (5.80%) being found in patients with diabetic nephropathy. No significant correlations were found between glycated apo B and the traditional parameters of glycemic control, such as glycated hemoglobin and fructosamines, and direct influence by sudden plasma glucose fluctuations on apo B glycation was not shown either, perhaps for a low inherent glycability of this apolipoprotein.
It is unclear if these low proportions of glycated apo B in vivo may significantly affect lipoprotein metabolism assuming a pathophysiological role in atherogenesis.
评估胰岛素依赖型和非胰岛素依赖型糖尿病患者载脂蛋白B的非酶糖基化水平。
采用亲和色谱法和免疫比浊法相结合的方法，我们测定了140例糖尿病患者（43例胰岛素依赖型糖尿病患者和97例非胰岛素依赖型糖尿病患者）以及45例非糖尿病对照者血清中糖化载脂蛋白B（apo B）的浓度。
虽然仅在非胰岛素依赖型糖尿病患者中血清总apo B浓度显著升高，但两组糖尿病患者的糖化apo B百分比（胰岛素依赖型糖尿病患者为4.84±0.8%；非胰岛素依赖型糖尿病患者为5.61±1.1%）均高于对照者（4.28±1.0%），其中糖尿病肾病患者的百分比最高（5.80%）。未发现糖化apo B与传统血糖控制参数（如糖化血红蛋白和果糖胺）之间存在显著相关性，也未显示血浆葡萄糖突然波动对apo B糖基化有直接影响，这可能是因为该载脂蛋白的固有糖基化能力较低。
尚不清楚体内这些低比例的糖化apo B是否会在动脉粥样硬化的病理生理过程中发挥作用，从而显著影响脂蛋白代谢。
