Witz C A, Montoya I A, Dey T D, Schenken R S
Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio 78284-7836.
Am J Reprod Immunol. 1994 Oct;32(3):173-9. doi: 10.1111/j.1600-0897.1994.tb01110.x.
Numerous studies have characterized the lymphocyte subpopulations in normal eutopic endometrium and suggested a role for the cytokine secretory products of these lymphocytes in regulating endometrial cell proliferation and differentiation. Recent studies have shown that ectopic endometrium contains a greater concentration of scattered stromal lymphocytes than does eutopic endometrium. However, the lymphocyte subpopulations and their activation status have not been characterized in ectopic endometrium.
We performed immunohistochemical studies on serial sections of proliferative and secretory phase eutopic endometrium and ectopic endometrium obtained during the proliferative phase using monoclonal antibodies to CD4 (T helper-inducer cells), CD8 (T cytolytic-suppressor cells), CD22 (B-cells), CD56 (natural killer cells), and VLA-1 (T-cell activation marker).
Ectopic endometrium contained significantly more scattered stromal CD4, CD8, and activated T cells than did proliferative and secretory eutopic endometrium. There were more activated T-cells in proliferative than in secretory eutopic endometrium. Ectopic endometrium contained significantly fewer NK cells than proliferative and secretory endometrium.
These results demonstrate that (1) the increased lymphocyte population in ectopic endometrium is due to increased numbers of CD4 and CD8 cells, and (2) a greater number of activated T cells are present in ectopic endometrium as compared to eutopic endometrium. Increased concentration of stromal T cells and enhanced VLA-1 expression in ectopic endometrium suggest that cytokine products of the activated T-cells may be involved in regulating cellular processes of endometriosis tissue.
众多研究已对正常在位内膜中的淋巴细胞亚群进行了特征描述，并表明这些淋巴细胞分泌的细胞因子产物在调节内膜细胞增殖和分化中发挥作用。最近的研究表明，异位内膜中散在的基质淋巴细胞浓度高于在位内膜。然而，异位内膜中的淋巴细胞亚群及其激活状态尚未得到特征描述。
我们使用针对CD4（辅助性诱导T细胞）、CD8（细胞毒性抑制性T细胞）、CD22（B细胞）、CD56（自然杀伤细胞）和VLA-1（T细胞激活标志物）的单克隆抗体，对增殖期和分泌期在位内膜以及增殖期获取的异位内膜的连续切片进行了免疫组织化学研究。
异位内膜中散在的基质CD4、CD8和活化T细胞明显多于增殖期和分泌期的在位内膜。增殖期在位内膜中的活化T细胞多于分泌期在位内膜。异位内膜中的自然杀伤细胞明显少于增殖期和分泌期内膜。
这些结果表明：（1）异位内膜中淋巴细胞数量增加是由于CD4和CD8细胞数量增多；（2）与在位内膜相比，异位内膜中存在更多的活化T细胞。异位内膜中基质T细胞浓度增加以及VLA-1表达增强表明，活化T细胞的细胞因子产物可能参与调节子宫内膜异位症组织的细胞过程。
