Sugisaki T, Yamada T, Saitoh S, Takamatsu K, Kubota C, Noguchi T
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Brain Res. 1994 Nov 28;665(1):101-6. doi: 10.1016/0006-8993(94)91157-6.
To determine factors contributing to the expression of the brain-derived protein, hippocalcin, we mapped its distribution in the brain of Snell pituitary dwarf mutant mice (dw) by immunohistochemical and immunoblot methods. Our findings are as follows. (1) In the hippocampus, hippocalcin immunoreactivity was found in the cell body and dendrites of pyramidal neurons of the normal controls and dw mice, although the intensity of immunoreactivity in the dw mice was lower. (2) In the cerebellum, hippocalcin immunoreactivity was strongly expressed in the Purkinje cell body of both the control and dw mice. However, the Purkinje cell dendrites were found to be more intensely stained in the dw mice than in the normal controls. (3) In the dw cerebral cortex, the pyramidal neurons of layers II to VI strongly expressed hippocalcin, whereas its expression in the controls was weak. (4) The amount of hippocalcin in the dw hippocampus was less than in the normal controls, whereas the amount in the dw cerebral cortex and cerebellum was greater. These results indicate that the developmental expression of hippocalcin in the dw brain is affected by the retarded maturation of the neuronal network due to the deficient hormonal state (the lack of growth and thyroid hormones).
为了确定影响脑源性蛋白海马钙素表达的因素，我们通过免疫组织化学和免疫印迹方法绘制了其在斯内尔垂体侏儒突变小鼠（dw）大脑中的分布情况。我们的研究结果如下。（1）在海马体中，正常对照小鼠和dw小鼠的锥体神经元细胞体和树突中均发现了海马钙素免疫反应性，尽管dw小鼠的免疫反应强度较低。（2）在小脑中，对照小鼠和dw小鼠的浦肯野细胞体中均强烈表达海马钙素免疫反应性。然而，发现dw小鼠的浦肯野细胞树突比正常对照小鼠染色更强烈。（3）在dw小鼠的大脑皮质中，II至VI层的锥体神经元强烈表达海马钙素，而在对照小鼠中其表达较弱。（4）dw小鼠海马体中的海马钙素含量低于正常对照小鼠，而dw小鼠大脑皮质和小脑中的含量则更高。这些结果表明，由于激素状态不足（缺乏生长激素和甲状腺激素）导致神经元网络成熟延迟，dw小鼠大脑中海马钙素的发育表达受到影响。
