[NMDA受体与长时程增强]

[NMDA receptor and long-term potentiation].

作者信息

Gozlan H, Diabira D, Chinestra P, Ben-Ari Y

机构信息

INSERM U. 29, Hôpital de Port-Royal, Paris, France.

出版信息

C R Acad Sci III. 1994 Oct;317(10):885-90.

PMID:7882133

Abstract

In order to evaluate the role of the NMDA receptor redox site in long-term potentiation (LTP), we have investigated the effects of two redox reagents, 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and tris(carboxyethyl)phosphine (TCEP) on the induction and expression of various forms of LTP. DTNB a thiol-oxidizing agent, irreversibly reduces by 50% NMDA receptor EPSP. In the presence of DTNB, the induction of tetanic and anoxic LTP are prevented. When tetanic or anoxic LTP were generated first, DTNB completely reverses the potentiation and TCEP a disulfide-reducing agent restores LTP to its initial level. These redox agents have no effect on AMPA synaptic transmission and did not significantly modify the induction and the expression of tetanic AMPA-LTP. These results suggest that thiol-oxidizing compounds might be useful for the treatment of cerebral ischemia.

摘要

为了评估N-甲基-D-天冬氨酸（NMDA）受体氧化还原位点在长时程增强（LTP）中的作用，我们研究了两种氧化还原试剂，5,5'-二硫代双-2-硝基苯甲酸（DTNB）和三（羧乙基）膦（TCEP）对各种形式LTP的诱导和表达的影响。DTNB是一种硫醇氧化剂，可使NMDA受体兴奋性突触后电位（EPSP）不可逆地降低50%。在DTNB存在的情况下，强直刺激和缺氧诱导的LTP被阻止。当先产生强直刺激或缺氧诱导的LTP时，DTNB可完全逆转这种增强作用，而二硫键还原剂TCEP可使LTP恢复到初始水平。这些氧化还原试剂对α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）突触传递没有影响，也没有显著改变强直刺激诱导的AMPA-LTP的诱导和表达。这些结果表明，硫醇氧化化合物可能对治疗脑缺血有用。