Zarate-Osorno A, Roman L N, Kingma D W, Meneses-Garcia A, Jaffe E S
Department of Pathology, Hospital Central Militar, Mexico City, Mexico.
Cancer. 1995 Mar 15;75(6):1360-6. doi: 10.1002/1097-0142(19950315)75:6<1360::aid-cncr2820750619>3.0.co;2-u.
The Epstein-Barr virus (EBV) has been linked to several human malignancies, including Hodgkin's disease (HD). In addition, epidemiologic studies have shown differences in HD occurrence in different parts of the world. The authors studied 27 cases of Hodgkin's disease from Mexico to determine the prevalence of EBV in HD in this developing nation.
The Epstein-Barr virus was investigated using in situ hybridization with the EBER1 probe. Immunohistochemical studies were performed on paraffin sections. Cases from both adult and pediatric age groups were included. Correlations with histologic subtype, clinicopathologic features, and immunophenotype were determined.
Epstein-Barr virus sequences were identified in 18/27 (67%) cases. Positivity correlated with histologic subtype: 0/1 lymphocyte predominant; 6/13 (46%) nodular sclerosis; 7/7 mixed cellularity (MC) (100%); and 5/6 (83%) lymphocyte depleted (LD). The proportion of cases classified as MC and LD (13 of 27) was greater than that found in the United States and other developed countries. The immunophenotypic profile was appropriate for Hodgkin's disease, with all cases of classic Hodgkin's disease positive for CD30 (Ber-H2) and 18 cases expressing CD15. One case of lymphocyte-predominant Hodgkin's disease was CD20 (L26)-positive as were three cases of classic Hodgkin's disease. Patient age ranged from 5 to 65 years, with a median of 29 years.
The EBV is associated highly with HD in Mexico, and this prevalence rate is found in all age groups. A strong correlation between EBV expression and histologic subtype was confirmed, with 92% of MC and LD subtypes found to be positive.
爱泼斯坦-巴尔病毒（EBV）与多种人类恶性肿瘤有关，包括霍奇金淋巴瘤（HD）。此外，流行病学研究表明，HD在世界不同地区的发病率存在差异。作者对来自墨西哥的27例霍奇金淋巴瘤患者进行研究，以确定这种发展中国家HD中EBV的感染率。
采用EBER1探针原位杂交法检测爱泼斯坦-巴尔病毒。对石蜡切片进行免疫组织化学研究。纳入成人和儿童年龄组的病例。确定与组织学亚型、临床病理特征和免疫表型的相关性。
27例中有18例（67%）检测到爱泼斯坦-巴尔病毒序列。阳性与组织学亚型相关：淋巴细胞为主型0/1；结节硬化型6/13（46%）；混合细胞型（MC）7/7（100%）；淋巴细胞消减型（LD）5/6（83%）。分类为MC和LD的病例比例（27例中的13例）高于美国和其他发达国家。免疫表型符合霍奇金淋巴瘤，所有经典霍奇金淋巴瘤病例CD30（Ber-H2）阳性，18例表达CD15。1例淋巴细胞为主型霍奇金淋巴瘤CD20（L26）阳性，3例经典霍奇金淋巴瘤也呈阳性。患者年龄从5岁到65岁不等，中位数为29岁。
在墨西哥，EBV与HD高度相关，且在所有年龄组中均有此感染率。证实EBV表达与组织学亚型之间存在强相关性，MC和LD亚型中92%呈阳性。
