Paolucci M, Di Fiore M M
Department of Zoology, Università degli Studi di Napoli, Federico II, Italy.
Gen Comp Endocrinol. 1994 Dec;96(3):401-11. doi: 10.1006/gcen.1994.1196.
Sex steroid binding proteins (SSBP) have been reported in the plasma of female and male Rana esculenta. In both sexes SSBP bind [3H]estradiol and [3H]testosterone with medium-high affinity and high specificity. Using ion-exchange chromatography SSBP resolve into two peaks eluting at 0.27 M (peak I) and 0.36 M (peak II) NaCl. Both peaks bind [3H]estradiol and [3H]testosterone equally well. Isoelectrofocusing showed that peak I focused at pH 6.0 and 7.5, whereas peak II focused at pH 6.0. SSBP capacity for [3H]estradiol and [3H]testosterone changes throughout the reproductive cycle, showing low levels during the nonbreeding period and increasing levels during the breeding period. Hypophysectomy and/or gonadectomy result in small changes in SSBP capacity. Short-term steroid hormone treatment of gonadectomized animals does not modify SSBP capacity.
在食用蛙的雌性和雄性血浆中均已报道存在性类固醇结合蛋白（SSBP）。在两性中，SSBP均以中等偏高的亲和力和高特异性结合[3H]雌二醇和[3H]睾酮。使用离子交换色谱法，SSBP可分离为两个峰，分别在0.27M（峰I）和0.36M（峰II）NaCl浓度下洗脱。两个峰结合[3H]雌二醇和[3H]睾酮的能力相当。等电聚焦显示，峰I聚焦在pH 6.0和7.5，而峰II聚焦在pH 6.0。SSBP结合[3H]雌二醇和[3H]睾酮的能力在整个生殖周期中都会发生变化，在非繁殖期水平较低，在繁殖期水平升高。垂体切除和/或性腺切除导致SSBP能力发生微小变化。对性腺切除动物进行短期类固醇激素治疗不会改变SSBP能力。
