人类芳烃受体：功能表达及染色体定位至7p21。

Human arylhydrocarbon receptor: functional expression and chromosomal assignment to 7p21.

作者信息

Ema M, Matsushita N, Sogawa K, Ariyama T, Inazawa J, Nemoto T, Ota M, Oshimura M, Fujii-Kuriyama Y

机构信息

Department of Chemistry, Faculty of Science, Tohoku University, Sendai.

出版信息

J Biochem. 1994 Oct;116(4):845-51. doi: 10.1093/oxfordjournals.jbchem.a124605.

DOI:10.1093/oxfordjournals.jbchem.a124605

PMID:7883760

Abstract

We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. The overall amino acid identity with mouse AhR from cell line Hepa-1 is 72.5%. The human AhR expressed either in COS-7 cells or in a reticulocyte lysate in vitro translation system showed specific dioxin-binding activity and Arnt-dependent DNA-binding activity. Chromosomal localization of the AhR gene was determined to be chromosome 7p21 by fluorescent in situ hybridization and DNA blot hybridization using 23 human x mouse or Chinese hamster hybrid cell DNAs.

摘要

我们以小鼠芳烃受体（AhR）cDNA作为标记探针，从人肺cDNA文库中分离出了人芳烃受体（AhR）cDNA。克隆得到的人AhR cDNA的核苷酸序列与先前报道的源自HepG2细胞系的人AhR序列[Dolwick等人（1993年），《分子药理学》44卷，911 - 917页]相同。与源自Hepa - 1细胞系的小鼠AhR的总体氨基酸同一性为72.5%。在COS - 7细胞中或在网织红细胞裂解物体外翻译系统中表达的人AhR表现出特异性二噁英结合活性和依赖于芳烃核转运蛋白（Arnt）的DNA结合活性。通过使用23种人×小鼠或中国仓鼠杂交细胞DNA进行荧光原位杂交和DNA印迹杂交，确定AhR基因的染色体定位为7号染色体p21区域。