Garcia C K, Li X, Luna J, Francke U
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235-9046.
Genomics. 1994 Sep 15;23(2):500-3. doi: 10.1006/geno.1994.1532.
Lactate and pyruvate are transported across cell membranes by monocarboxylate transporters (MCTs). Here, we use the recently cloned cDNA for hamster MCT1 to isolate cDNA and genomic clones for human MCT1. Comparison of the human and hamster amino acid sequences revealed that the proteins are 86% identical. The gene for human MCT1 (gene symbol, SLC16A1) was localized to human chromosome bands 1p13.2-p12 by PCR analysis of panels of human x rodent cell hybrid lines and by fluorescence chromosomal in situ hybridization.
乳酸和丙酮酸通过单羧酸转运体（MCTs）跨细胞膜转运。在此，我们利用最近克隆的仓鼠MCT1的互补DNA（cDNA）来分离人类MCT1的cDNA和基因组克隆。对人类和仓鼠氨基酸序列的比较显示，这两种蛋白质的同源性为86%。通过对人类×啮齿动物细胞杂交系的PCR分析以及荧光染色体原位杂交，将人类MCT1基因（基因符号，SLC16A1）定位到人类染色体1p13.2 - p12区域。
