D'Andrea E, Baffa R, Menin C, Montagna M, Rugge M, Chieco-Bianchi L
Institute of Oncology, Interuniversity Center for Research on Cancer, Padora, Italy.
J Cancer Res Clin Oncol. 1995;121(2):79-83. doi: 10.1007/BF01202217.
TP53 gene mutations, one of the most common alterations described in human tumors, have also been detected in gastric carcinoma, and shown to occur rather late in disease progression. A better assessment of the prognostic value of TP53 gene mutations can be obtained by examining archival material, as this allows stored cases with well-defined histories to be monitored. We performed immunohistochemical and polymerase chain reaction/single-strand conformation polymorphism (PCR-SSCP) analyses of formalin-fixed paraffin-embedded material from nine selected cases of gastric carcinoma at different pathological stages. PCR-SSCP analysis of TP53 exons 5-8 detected missense point mutations in two out of five immunostain(PAb1801)-positive tumors, and a deletion (allowing for a premature stop codon) in one of the remaining four immunostain-negative tumors. Thus, PCR-SSCP analysis represents a feasible strategy for the detection of TP53 alterations in archival material of gastric carcinoma cases.
TP53基因突变是人类肿瘤中描述的最常见改变之一，在胃癌中也已被检测到，并且显示在疾病进展过程中出现得较晚。通过检查存档材料可以更好地评估TP53基因突变的预后价值，因为这可以对病史明确的储存病例进行监测。我们对9例不同病理阶段的胃癌病例的福尔马林固定石蜡包埋材料进行了免疫组织化学和聚合酶链反应/单链构象多态性（PCR-SSCP）分析。对TP53基因第5至8外显子的PCR-SSCP分析在5例免疫染色（PAb1801）阳性肿瘤中的2例中检测到错义点突变，在其余4例免疫染色阴性肿瘤中的1例中检测到缺失（导致提前终止密码子）。因此，PCR-SSCP分析是检测胃癌病例存档材料中TP53改变的一种可行策略。
