Mansour A, Burke S, Pavlic R J, Akil H, Watson S J
Mental Health Research Institute, University of Michigan, Ann Arbor, USA.
Neuroscience. 1996 Apr;71(3):671-90. doi: 10.1016/0306-4522(95)00464-5.
Several lines of evidence have demonstrated the presence of three opioid receptor types in the CNS and periphery. These receptors are referred to as mu, delta and kappa, and have been implicated in a wide variety of functions. The present study examines the localization of the kappa 1 receptor, a region of the receptor that has little homology with mu and delta receptors. Immunohistochemical studies in Zamboni-fixed rat tissue demonstrate immunoreactive perikarya and/or fibers in such regions as the deep layers of the parietal, temporal and occipital cortex, parasubiculum, central and medial amygdala, bed nucleus stria terminalis, nucleus accumbens, olfactory tubercle, endopiriform nucleus, claustrum, hypothalamic nuclei, median eminence, midline thalamic nuclei, zona incerta, central gray, caudal linear and dorsal raphe, substantia nigra, pars reticulata, ventral tegmental area, parabrachial nucleus, spinal trigeminal nucleus, nucleus of the solitary tract, spinal cord and the dorsal root ganglia. Specific kappa 1 receptor-like immunohistochemical staining is also observed in the pituitary, where immunoreactive perikarya and fibers are localized in the neural and intermediate lobes. Transfection and preabsorption controls suggest that the antibody is selective for the cloned kappa 1 receptor, and does not recognize mu or delta. This immunohistochemical localization corresponds well to previously described kappa 1 receptor mRNA and binding distributions and provides new insights into the cellular localization and pre- and postsynaptic organization of the kappa 1 receptor-like proteins in the rat brain and pituitary. The functional implications of these results are discussed in light of the kappa 1 receptors play in hormonal regulation, antinociception and reward.
多条证据表明，中枢神经系统和外周存在三种阿片受体类型。这些受体被称为μ、δ和κ受体，并且与多种功能有关。本研究检测κ1受体的定位，该受体区域与μ和δ受体几乎没有同源性。对用赞博尼固定液固定的大鼠组织进行免疫组织化学研究显示，在顶叶、颞叶和枕叶皮质深层、副海马体、中央杏仁核和内侧杏仁核、终纹床核、伏隔核、嗅结节、内梨状核、屏状核、下丘脑核、正中隆起、丘脑中线核、未定带、中央灰质、尾侧线性核和背侧中缝核、黑质网状部、腹侧被盖区、臂旁核、三叉神经脊束核、孤束核、脊髓以及背根神经节等区域存在免疫反应性核周体和/或纤维。在垂体中也观察到特异性κ1受体样免疫组织化学染色，其中免疫反应性核周体和纤维定位于神经叶和中间叶。转染和预吸收对照表明，该抗体对克隆的κ1受体具有选择性，不识别μ或δ受体。这种免疫组织化学定位与先前描述的κ1受体mRNA和结合分布非常吻合，并为大鼠脑和垂体中κ1受体样蛋白的细胞定位以及突触前和突触后组织提供了新的见解。根据κ1受体在激素调节、抗伤害感受和奖赏中的作用，讨论了这些结果的功能意义。
