大鼠脊髓和背根神经节中μ-和κ-阿片受体mRNA的原位杂交研究

In situ hybridization study of mu- and kappa-opioid receptor mRNAs in the rat spinal cord and dorsal root ganglia.

作者信息

Maekawa K, Minami M, Yabuuchi K, Toya T, Katao Y, Hosoi Y, Onogi T, Satoh M

机构信息

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.

出版信息

Neurosci Lett. 1994 Feb 28;168(1-2):97-100. doi: 10.1016/0304-3940(94)90425-1.

DOI:10.1016/0304-3940(94)90425-1

PMID:8028801

Abstract

Distributions of mu- and kappa-opioid receptor mRNAs in the lumbar spinal cord and dorsal root ganglia of the adult rat were examined using the in situ hybridization technique. In the lumbar spinal cord, mu-opioid receptor mRNA was expressed intensely in laminae I, II and VIII. On the other hand, kappa-opioid receptor mRNA was expressed intensely in laminae I and II, and moderately throughout laminae III-VIII. In the dorsal root ganglia, mu-opioid receptor mRNA was intensely expressed and kappa-opioid receptor mRNA was expressed in a smaller number of cells than mu-opioid receptor mRNA.

摘要

采用原位杂交技术检测成年大鼠腰段脊髓和背根神经节中μ-阿片受体和κ-阿片受体mRNA的分布。在腰段脊髓中，μ-阿片受体mRNA在I、II和VIII层强烈表达。另一方面，κ-阿片受体mRNA在I和II层强烈表达，在III-VIII层中度表达。在背根神经节中，μ-阿片受体mRNA强烈表达，κ-阿片受体mRNA表达的细胞数量少于μ-阿片受体mRNA。