Ichinose T, Miller M G, Shibamoto T
Department of Environmental Toxicology, University of California, Davis 95616.
J Appl Toxicol. 1994 Nov-Dec;14(6):453-5. doi: 10.1002/jat.2550140611.
Free malonaldehyde formed in the microsomes prepared from livers of monkey, rat, rabbit, mouse, cow, pig, dog, sheep and horse upon CCl4 oxidation was derivatized by reaction with N-methylhydrazine to form 1-methylpyrazole which was subsequently analyzed by capillary gas chromatography. Among the livers from animals tested, the monkey and rat livers produced the most malonaldehyde upon CCl4 treatment. Horse liver showed the greatest resistance to CCl4 oxidation. The gas chromatography method used in the present study exhibited an accurate and specific measurement of free malonaldehyde that might provide an understanding of the biochemical process of in vitro lipid peroxidation.
用四氯化碳氧化从猴、大鼠、兔、小鼠、牛、猪、狗、羊和马的肝脏制备的微粒体中形成的游离丙二醛，通过与N-甲基肼反应进行衍生化，形成1-甲基吡唑，随后通过毛细管气相色谱法进行分析。在所测试动物的肝脏中，猴肝和大鼠肝在四氯化碳处理后产生的丙二醛最多。马肝对四氯化碳氧化表现出最大的抗性。本研究中使用的气相色谱法对游离丙二醛进行了准确而特异的测量，这可能有助于理解体外脂质过氧化的生化过程。
