Mukherjee S S, Murthi P S, Mukherjee S K
Acta Diabetol Lat. 1976 Jan-Apr;13(1-2):8-19. doi: 10.1007/BF02591576.
The effect of centpiperalone [2-piperazino-4(3H)-quinazolinone monoacetate], a potent hypoglycemic compound on glucose and oxygen uptake, and lactic acid formation by rat hemidiaphragms suspended in glucose-phosphate buffer in Warburg bath at 37 degrees C and 80 shakes/min was studied in vitro. Although centpiperalone enhanced glucose and oxygen uptake by itself, no increment in the above parameters was seen when insulin was added simultaneously along with centpiperalone. Preincubation of hemidiaphragms with centpiperalone and their subsequent incubation with insulin did not indicate any potentiation of insulin action as indicated by uptake of oxygen and glucose. However when 16 times diluted serum from centpiperalone-treated rats was added to hemidiaphragms suspended in glucose-phosphate buffer, significant increase in glycogen content was observed compared to that seen in hemidiaphragms after addition of serum from normal untreated and alloxanized centpiperalone-fed rats. Definite degranulation of B-cells was seen in centpiperalone-fed rats using the aldehyde fuchsin staining technique. These data strongly suggest that centpiperalone induces hypoglycemia through liberation of insulin from B-cells.
在体外研究了强效降糖化合物胡椒嗪酮[2-哌嗪基-4(3H)-喹唑啉酮单乙酸酯]对葡萄糖摄取、氧摄取以及在37℃和每分钟80次振荡条件下悬浮于Warburg浴中葡萄糖-磷酸盐缓冲液中的大鼠半膈肌乳酸生成的影响。尽管胡椒嗪酮自身可增强葡萄糖摄取和氧摄取，但当它与胰岛素同时添加时，上述参数未见增加。半膈肌先用胡椒嗪酮预孵育，随后再用胰岛素孵育，结果显示，氧摄取和葡萄糖摄取未表明胰岛素作用有任何增强。然而，当将经胡椒嗪酮处理的大鼠的血清稀释16倍后加入悬浮于葡萄糖-磷酸盐缓冲液中的半膈肌时，与加入正常未处理大鼠和经四氧嘧啶处理的胡椒嗪酮喂养大鼠的血清后的半膈肌相比，糖原含量显著增加。使用醛复红染色技术在经胡椒嗪酮喂养的大鼠中可见B细胞有明确的脱颗粒现象。这些数据有力地表明，胡椒嗪酮通过促使胰岛素从B细胞释放而诱发低血糖。
