绘制轮状病毒的血凝结构域图谱。
Mapping the hemagglutination domain of rotaviruses.
作者信息
Fuentes-Pananá E M, López S, Gorziglia M, Arias C F
机构信息
Departamento de Genética y Fisiología Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
出版信息
J Virol. 1995 Apr;69(4):2629-32. doi: 10.1128/JVI.69.4.2629-2632.1995.
Most strains of animal rotaviruses are able to agglutinate erythrocytes, and the surface protein VP4 is the virus hemagglutinin. To map the hemagglutination domain on VP4 while preserving the conformation of the protein, we constructed full-length chimeras between the VP4 genes of hemagglutinating (YM) and nonhemagglutinating (KU) rotavirus strains. The parental and chimeric genes were expressed in insect cells, and the recombinant VP4 proteins were evaluated for their capacity to agglutinate human type O erythrocytes. Three chimeric genes, encoding amino acids 1 to 208 (QKU), 93 to 208 (QC), and 93 to 776 (QYM) of the YM VP4 protein in a KU VP4 background, were constructed. YM VP4 and chimeras QKU and QC were shown to specifically hemagglutinate, indicating that the region between amino acids 93 and 208 of YM VP4 is sufficient to determine the hemagglutination activity of the protein.
大多数动物轮状病毒毒株能够凝集红细胞，表面蛋白VP4是病毒血凝素。为了在保留蛋白质构象的同时绘制VP4上的血凝结构域，我们构建了血凝性（YM）和非血凝性（KU）轮状病毒毒株VP4基因之间的全长嵌合体。亲本基因和嵌合基因在昆虫细胞中表达，并评估重组VP4蛋白凝集人O型红细胞的能力。构建了三个嵌合基因，它们在KU VP4背景中编码YM VP4蛋白的第1至208位氨基酸（QKU）、第93至208位氨基酸（QC）和第93至776位氨基酸（QYM）。结果显示YM VP4以及嵌合体QKU和QC具有特异性血凝作用，表明YM VP4第93至208位氨基酸之间的区域足以决定该蛋白的血凝活性。
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