胰蛋白酶活性丧失导致多周期流感病毒在Vero（世卫组织）细胞中的生长受损。
Impairment of multicycle influenza virus growth in Vero (WHO) cells by loss of trypsin activity.
作者信息
Kaverin N V, Webster R G
机构信息
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
出版信息
J Virol. 1995 Apr;69(4):2700-3. doi: 10.1128/JVI.69.4.2700-2703.1995.
Abstract
We demonstrated that influenza virus replication in Vero (WHO) cells, a subline of African green monkey kidney cells, is impaired by rapid inactivation of trypsin in the culture fluids. Trypsin inactivation was caused by a factor secreted by Vero cells into the media. Repeated addition of trypsin to the culture medium of influenza virus-infected Vero cells restores the multicycle growth pattern of influenza A virus strains, allowing high yields to be obtained at a low multiplicity of infection. These findings may permit efficient use of Vero (WHO) cells in the production of influenza vaccines.
摘要
我们证明，在非洲绿猴肾细胞的一个亚系Vero（世卫组织）细胞中，流感病毒的复制会因培养液中胰蛋白酶的快速失活而受到损害。胰蛋白酶失活是由Vero细胞分泌到培养基中的一种因子引起的。向感染流感病毒的Vero细胞培养基中反复添加胰蛋白酶，可恢复甲型流感病毒株的多循环生长模式，从而在低感染复数下获得高产量。这些发现可能有助于在流感疫苗生产中有效利用Vero（世卫组织）细胞。
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