Conti G, Valcavi P, Natali A, Schito G C
Arch Virol. 1980;66(4):309-20. doi: 10.1007/BF01320627.
When KB cells were infected either with the fowl plague (FPV) Rostock strain (Hav1N1) or the WSN (H0N1) strain of influenza A virus the yield of cell-associated haemagglutinin and neuraminidase polypeptides was essentially comparable, but virus particles were not produced in the FPV-KB system. WSN virus-infected KB cells synthesized normal amounts of mature virus particles and had all the characteristics of a permissive replication cycle. Biosynthesis and transport of RNP antigen from nucleus to cytoplasm of infected cells were traced by immunofluorescent staining at 4 and 8 hours after the beginning of infection. While the fluorescent-stained material was totally confined to the nuclei in FPV-infected KB cells, RNP antigen migrated out of the nucleus during the replicative cycle of WSN virus in the same host cell. Patterns of virus-specific protein synthesis were studied by pulse-labelling with 35S-methionine. The most significant feature concerned the amplification of synthesis of virus-induced matrix (M) protein which did not occur in FPV-infected cells but occurred normally during WSN infection. The different patterns of replication in the same host cell when infected by different influenza A viruses is discussed.
当KB细胞感染禽瘟（FPV）罗斯托克株（Hav1N1）或甲型流感病毒WSN（H0N1）株时，细胞相关血凝素和神经氨酸酶多肽的产量基本相当，但在FPV-KB系统中未产生病毒颗粒。WSN病毒感染的KB细胞合成了正常数量的成熟病毒颗粒，并具有允许复制周期的所有特征。在感染开始后4小时和8小时，通过免疫荧光染色追踪感染细胞中RNP抗原从细胞核到细胞质的生物合成和运输。在FPV感染的KB细胞中，荧光染色物质完全局限于细胞核内，而在同一宿主细胞中，WSN病毒复制周期期间，RNP抗原从细胞核中迁移出来。通过用35S-甲硫氨酸脉冲标记研究了病毒特异性蛋白质合成模式。最显著的特征是病毒诱导的基质（M）蛋白合成的扩增，这在FPV感染的细胞中未发生，但在WSN感染期间正常发生。讨论了同一宿主细胞被不同甲型流感病毒感染时的不同复制模式。
