Zhu B, Gu A, Deng X, Geng Y, Lu Z
Institute of Genetics, Chinese Academy of Sciences, Beijing.
Mutat Res. 1995 Apr;334(2):157-9.
Seeds of soybean cultivar LD4 were mutagenically treated with EMS (0.3, 0.5, 0.6, 0.9, 1.5 and 1.8%) for 3 h only or plus caffeine (50 mM) or EDTA (1 mM) post-treatment for 5 h. The experimental results indicated that: (1) of the different concentrations of EMS treatment, the M2 mutation frequency induced with 0.6% EMS was the highest (9.7%). When the EMS concentration was over 0.9%, the mutation frequency decreased rapidly. (2) Of the EMS treatments plus caffeine or EDTA post-treatment, the mutagenic effect of 0.6% EMS was the best for inducing morphological variations. Caffeine post-treatment decreased notably the mutation frequency of EMS treatment; when concentrations of EMS were very high (1.5% and 1.8%), mutation frequencies of EDTA post-treatment were still 5.0% and 4.88%, but no mutants were found in EMS treatment or plus caffeine post-treatment. (3) In the M2 mutation spectrum, 11 kinds of mutant types were observed in EMS treatment or plus caffeine or EDTA post-treatment. Relative frequencies of some mutant types (growth period, plant height, grain size, leaf shape and sterility, etc.) were similar among the three treatments, but EDTA post-treatment could change the relative frequencies of yield characteristics (number of pods and grains, grain weight/plant) induced by EMS treatment only.
用EMS（0.3%、0.5%、0.6%、0.9%、1.5%和1.8%）对大豆品种LD4的种子进行诱变处理3小时，或在处理后加咖啡因（50 mM）或EDTA（1 mM）再处理5小时。实验结果表明：（1）在不同浓度的EMS处理中，0.6% EMS诱导的M2突变频率最高（9.7%）。当EMS浓度超过0.9%时，突变频率迅速下降。（2）在EMS处理后加咖啡因或EDTA的处理中，0.6% EMS的诱变效果对诱导形态变异最佳。咖啡因后处理显著降低了EMS处理的突变频率；当EMS浓度非常高（1.5%和1.8%）时，EDTA后处理的突变频率仍分别为5.0%和4.88%，但在EMS处理或加咖啡因后处理中未发现突变体。（3）在M2突变谱中，在EMS处理或加咖啡因或EDTA后处理中观察到11种突变类型。三种处理中一些突变类型（生育期、株高、籽粒大小、叶形和不育等）的相对频率相似，但EDTA后处理只能改变EMS处理诱导的产量性状（荚数和粒数、单株粒重）的相对频率。
