Shah S N M, Gong Z-H, Arisha M H, Khan A, Tian S-L
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China
Genet Mol Res. 2015 Mar 30;14(1):2440-9. doi: 10.4238/2015.March.30.2.
We attempted to create a new germplasm of cucumber cultivar Chinese long (9930) using different doses of ethyl methyl sulfonate (EMS) to induce variability. We tested EMS concentration (0, 0.5, 1.0, 1.5, 2, 3% v/v) with post-treatment (0.1 M Na2S2O3 and water), EMS concentration (0, 0.5, 1.0, 1.5% v/v) over different treatment times (8, 16, 24 h), and EMS concentration (0, 0.5, 1.0, 1.5% v/v) with different treatment temperatures (20 and 28°C). In all experiments with increasing EMS concentration, germination percent, index, and rate were decreased. After addition of stop solution (0.1 M Na2S2O3), post-treatment mutated seeds showed higher germination (84.44%) and rate (37.5%) than seeds treated with water (80 and 34.07%, respectively), while the germination index was high in seeds treated with water. At 20°C, the germination index (4.13) and rate (56.25%) were affected to a greater extent than at 28°C (7.68 and 91.31%, respectively). Treatment times of 16 and 24 h showed similar results for germination percent and rate, while the germination index was decreased over time. There were significant differences in seedling height, fresh true leaf weight, seedling weight, and plant survival with increasing EMS concentration and time. Higher variations in the form of dwarf seedlings were recorded after treatment with 1.5% EMS for 24 h. Based on germination and morphological data, an EMS concentration of 1.5% for 24 h at 20°C and post-treatment with stop solution (0.1 M Na2S2O3) efficiently caused mutation.
我们试图使用不同剂量的甲基磺酸乙酯（EMS）诱导变异，从而创建一个新的黄瓜品种（9930）种质资源。我们测试了EMS浓度（0、0.5、1.0、1.5、2、3% v/v）及处理后（0.1 M Na2S2O3和水）的情况，不同处理时间（8、16、24小时）下的EMS浓度（0、0.5、1.0、1.5% v/v），以及不同处理温度（20和28°C）下的EMS浓度（0、0.5、1.0、1.5% v/v）。在所有实验中，随着EMS浓度的增加，发芽率、发芽指数和发芽速率均下降。添加终止溶液（0.1 M Na2S2O3）后，处理后的突变种子发芽率（84.44%）和发芽速率（37.5%）高于用水处理的种子（分别为80%和34.07%），而用水处理的种子发芽指数较高。在20°C时，发芽指数（4.13）和发芽速率（56.25%）受到的影响比在28°C时更大（分别为7.68和91.31%）。16和24小时的处理时间在发芽率和发芽速率方面显示出相似的结果，而发芽指数随时间下降。随着EMS浓度和处理时间的增加，幼苗高度、新鲜真叶重量、幼苗重量和植株存活率存在显著差异。用1.5% EMS处理24小时后，记录到更多矮化幼苗形式的变异。基于发芽和形态学数据，在20°C下用1.5% EMS处理24小时并使用终止溶液（0.1 M Na2S2O3）进行处理后能有效地诱导突变。
