Ferris N P, Oxtoby J M
Institute for Animal Health, Pirbright Laboratory, Surrey, UK.
Vet Microbiol. 1994 Nov;42(2-3):229-38. doi: 10.1016/0378-1135(94)90021-3.
An indirect, sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of antigens of vesicular exanthema, San Miguel sea lion viruses and other marine caliciviruses is described. The assay which uses rabbit and guinea-pig antisera to purified antigens of each calicivirus serotype has high sensitivity and is almost totally type-specific.
本文描述了一种间接夹心酶联免疫吸附测定法(ELISA),用于检测水疱性口炎、圣米格尔海狮病毒及其他海洋杯状病毒的抗原。该测定法使用兔和豚鼠抗血清针对每种杯状病毒血清型的纯化抗原,具有高灵敏度且几乎完全具有型特异性。