采用间接夹心多克隆酶联免疫吸附测定法诊断兔出血性疾病（RHD）和欧洲棕兔综合征（EBHS）

Diagnosis of rabbit haemorrhagic disease (RHD) and European brown hare syndrome (EBHS) by indirect sandwich polyclonal ELISA.

作者信息

Nardelli S, Agnoletti F, Costantini F, Parpajola R

机构信息

Istituto Zooprofilattico Sperimentale delle Vénezie, Padova, Italy.

出版信息

Zentralbl Veterinarmed B. 1996 Sep;43(7):393-400. doi: 10.1111/j.1439-0450.1996.tb00331.x.

DOI:10.1111/j.1439-0450.1996.tb00331.x

PMID:8885704

Abstract

The establishment of two indirect sandwich polyclonal ELISAs for the virological diagnosis of rabbit haemorrhagic disease (RHD) and European brown hare syndrome (EBHS) is described. Each assay uses rabbit and guinea pig antisera raised to RHD or EBHS purified virus particles. The tests are sensitive and specific, but cross-reactions between rabbit haemorrhagic disease virus (RHDV) and European brown hare syndrome virus (EBHSV) are observed. Most rabbit liver homogenates identified as RHDV-positive by ELISA do not haemagglutinate human red blood cells at room temperature.

摘要

本文描述了两种用于兔出血性疾病（RHD）和欧洲棕兔综合征（EBHS）病毒学诊断的间接夹心多克隆ELISA的建立。每种检测方法都使用针对RHD或EBHS纯化病毒颗粒产生的兔和豚鼠抗血清。这些检测方法灵敏且特异，但观察到兔出血性疾病病毒（RHDV）和欧洲棕兔综合征病毒（EBHSV）之间存在交叉反应。大多数经ELISA鉴定为RHDV阳性的兔肝匀浆在室温下不能凝集人红细胞。