大鼠磨牙牙槽骨中沙比纤维的电子显微镜研究。
Electron microscopic studies on Sharpey's fibers in the alveolar bone of rat molars.
作者信息
Kuroiwa M, Chihara K, Higashi S
机构信息
Second Department of Oral Anatomy, School of Dentistry, Showa University, Tokyo, Japan.
出版信息
Kaibogaku Zasshi. 1994 Dec;69(6):776-82.
The fine structure of Sharpey's fibers in the alveolar bone of rat molars were observed by electron microscope. The fixed samples were three-dimensionally observed with a scanning electron microscope (SEM) after NaOH treatment. The penetrating Sharpey's fibers in the alveolar bone appeared distinctly. The fibers penetrated straight and/or branched in the alveolar bone. The Sharpey's fiber bundles ran nearly perpendicular to the bone matrix fiber bundles. The other fixed samples were embedded in polyester resin, ground and observed by backscattered electron signals in an SEM (BSE). In the BSE images, even thin Sharpey's fibers deeply penetrating the alveolar bone were hypomineralized. The fine ultrastructure of fibers were also observed by a transmission electron microscope (TEM). It was suggested that the three-dimensional structure of Sharpey's fibers intensified the continuity between the periodontal ligament fiber and the alveolar bone and consisted of a buffer medium against stress.
用电子显微镜观察大鼠磨牙牙槽骨中沙比纤维的精细结构。固定后的样本经氢氧化钠处理后，用扫描电子显微镜（SEM）进行三维观察。牙槽骨中穿透的沙比纤维清晰可见。纤维在牙槽骨中笔直穿透和/或分支。沙比纤维束几乎垂直于骨基质纤维束。其他固定样本嵌入聚酯树脂中，研磨后用扫描电子显微镜（SEM）中的背散射电子信号进行观察。在背散射电子图像中，即使是深深穿透牙槽骨的细沙比纤维也矿化不足。还用透射电子显微镜（TEM）观察了纤维的精细超微结构。结果表明，沙比纤维的三维结构增强了牙周膜纤维与牙槽骨之间的连续性，并构成了一种应力缓冲介质。
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