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Wnt/β-连环蛋白信号通路抑制人牙周膜成纤维细胞的成骨分化。

Wnt/β-Catenin Signaling Inhibits Osteogenic Differentiation in Human Periodontal Ligament Fibroblasts.

作者信息

Iizumi Ryoya, Honda Michiyo

机构信息

Department of Applied Chemistry, School of Science and Technology, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki 214-8571, Kanagawa, Japan.

出版信息

Biomimetics (Basel). 2022 Dec 3;7(4):224. doi: 10.3390/biomimetics7040224.

DOI:10.3390/biomimetics7040224
PMID:36546925
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9776043/
Abstract

The periodontal ligament is a collagenous tissue that is important for maintaining the homeostasis of cementum and alveolar bone. In tendon cells, Wnt/β-catenin signaling has been reported to regulate the expression level of () and () gene and maintain the tissue homeostasis, while its role in the periodontal ligament is unclear. The aim of this study was to investigate the effects of Wnt/β-catenin signaling induced by Wnt-3a stimulation on the inhibition of osteogenic differentiation of human periodontal ligament fibroblasts (HPLFs). During osteogenic differentiation of HPLFs, they formed bone nodules independently of alkaline phosphatase (ALP) activity. After stimulation of Wnt-3a, the expression of β-catenin increased, and nuclear translocation of β-catenin was observed. These data indicate that Wnt-3a activated Wnt/β-catenin signaling. Furthermore, the stimulation of Wnt-3a inhibited the bone nodule formation and suppressed the expression of osteogenic differentiation-related genes such as , and , and upregulated the gene expression of and (). may be involved in the suppression of osteogenic differentiation in HPLFs. In conclusion, Wnt/β-catenin signaling may be an important signaling pathway that inhibits the osteogenic differentiation in HPLFs by the upregulation of gene expression and downregulation of osteogenic differentiation-related genes.

摘要

牙周韧带是一种胶原组织，对维持牙骨质和牙槽骨的稳态很重要。在肌腱细胞中，据报道Wnt/β-连环蛋白信号通路可调节（）和（）基因的表达水平并维持组织稳态，但其在牙周韧带中的作用尚不清楚。本研究的目的是探讨Wnt-3a刺激诱导的Wnt/β-连环蛋白信号通路对人牙周膜成纤维细胞（HPLFs）成骨分化抑制的影响。在HPLFs的成骨分化过程中，它们独立于碱性磷酸酶（ALP）活性形成骨结节。Wnt-3a刺激后，β-连环蛋白的表达增加，并观察到β-连环蛋白的核转位。这些数据表明Wnt-3a激活了Wnt/β-连环蛋白信号通路。此外，Wnt-3a刺激抑制了骨结节的形成，抑制了成骨分化相关基因如（）、（）和（）的表达，并上调了（）和（）的基因表达。（）可能参与了对HPLFs成骨分化的抑制。总之，Wnt/β-连环蛋白信号通路可能是一条重要的信号通路，通过上调（）基因表达和下调成骨分化相关基因来抑制HPLFs的成骨分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/31c99fd5dcca/biomimetics-07-00224-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/8c50e113e0d0/biomimetics-07-00224-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/65862799bf83/biomimetics-07-00224-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/42a766f99f31/biomimetics-07-00224-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/9defab3207ee/biomimetics-07-00224-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/da2c2ec9571f/biomimetics-07-00224-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/31c99fd5dcca/biomimetics-07-00224-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/8c50e113e0d0/biomimetics-07-00224-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/65862799bf83/biomimetics-07-00224-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/42a766f99f31/biomimetics-07-00224-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/9defab3207ee/biomimetics-07-00224-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/da2c2ec9571f/biomimetics-07-00224-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eefb/9776043/31c99fd5dcca/biomimetics-07-00224-g006.jpg

相似文献

[1]
Wnt/β-Catenin Signaling Inhibits Osteogenic Differentiation in Human Periodontal Ligament Fibroblasts.

Biomimetics (Basel). 2022-12-3

[2]
Activation of canonical Wnt/β-catenin signaling inhibits H2O2-induced decreases in proliferation and differentiation of human periodontal ligament fibroblasts.

Mol Cell Biochem. 2016-1

[3]
Crucial roles of canonical Runx2-dependent pathway on Wnt1-induced osteoblastic differentiation of human periodontal ligament fibroblasts.

Mol Cell Biochem. 2015-4

[4]
HOXA10 inhibit the osteogenic differentiation of periodontal ligament stem cells by regulating β-catenin localization and DKK1 expression.

Connect Tissue Res. 2021-7

[5]
Baicalein enhances the osteogenic differentiation of human periodontal ligament cells by activating the Wnt/β-catenin signaling pathway.

Arch Oral Biol. 2017-2-7

[6]
Berberine promotes bone marrow-derived mesenchymal stem cells osteogenic differentiation via canonical Wnt/β-catenin signaling pathway.

Toxicol Lett. 2016-1-5

[7]
Wnt/β-catenin pathway regulates bone morphogenetic protein (BMP2)-mediated differentiation of dental follicle cells.

J Periodontal Res. 2011-12-11

[8]
Luteolin supports osteogenic differentiation of human periodontal ligament cells.

BMC Oral Health. 2019-10-26

[9]
Human β-defensin 3-combined gold nanoparticles for enhancement of osteogenic differentiation of human periodontal ligament cells in inflammatory microenvironments.

Int J Nanomedicine. 2018-1-26

[10]
Salvianolic acid B promotes the osteogenic differentiation of human periodontal ligament cells through Wnt/β-catenin signaling pathway.

Arch Oral Biol. 2020-3-4

引用本文的文献

[1]
β-Catenin: A Key Molecule in Osteoblast Differentiation.

Biomolecules. 2025-7-18

[2]
Evaluating the link between periodontitis and oral squamous cell carcinoma through Wnt/β-catenin pathway: a critical review.

Front Oral Health. 2025-5-12

[3]
Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon.

J Orthop Translat. 2024-3-13

[4]
Wnt signaling in periodontitis.

Clin Oral Investig. 2023-11

本文引用的文献

[1]
Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development.

J Dev Biol. 2021-6-27

[2]
Analyses of key mRNAs and lncRNAs for different osteo-differentiation potentials of periodontal ligament stem cell and gingival mesenchymal stem cell.

J Cell Mol Med. 2021-5-24

[3]
Scleraxis upregulated by transforming growth factor-β1 signaling inhibits tension-induced osteoblast differentiation of priodontal ligament cells via ephrin A2.

Bone. 2021-8

[4]
Discovery and development of berberine derivatives as stimulants of osteoblast differentiation.

Biochem Biophys Res Commun. 2020-6-18

[5]
Wnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cells.

Cells. 2020-3-7

[6]
Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet.

Sci Rep. 2020-2-3

[7]
Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells.

BMC Dev Biol. 2020-1-14

[8]
CB1 enhanced the osteo/dentinogenic differentiation ability of periodontal ligament stem cells via p38 MAPK and JNK in an inflammatory environment.

Cell Prolif. 2019-10-10

[9]
Requirement for scleraxis in the recruitment of mesenchymal progenitors during embryonic tendon elongation.

Development. 2019-10-4

[10]
Periodontal Bone-Ligament-Cementum Regeneration via Scaffolds and Stem Cells.

Cells. 2019-6-4

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