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拟杆菌的化学分类学综述

Chemotaxonomy of Bacteroides: a review.

作者信息

Olsen I

机构信息

Department of Oral Biology, Faculty of Dentistry, University of Oslo, Norway.

出版信息

Acta Odontol Scand. 1994 Dec;52(6):354-67. doi: 10.3109/00016359409029033.

DOI:10.3109/00016359409029033
PMID:7887145
Abstract

The loose definition of Bacteroides, some species of which are important etiologic agents of oral diseases, has enabled isolates with only marginal similarities to be reposited in this genus. Many attempts have been made over the years to improve the taxonomy of this heterogeneous group of bacteria. The present article reviews major chemotaxonomic characters and techniques that have been used for this purpose: pigmentation, metabolites, whole-cell fatty acids, phospholipids, isoprenoid quinones, carbohydrates of lipopolysaccharide, whole-cell proteins, peptidoglycans, enzymes, pyrolysis mass spectrometry, DNA composition, restriction fragment length polymorphisms of DNA and ribosomal (r) RNA, homology of DNA and RNA, DNA-rRNA hybridization, and 16S and 5S rRNA oligonucleotide cataloging and sequencing. Despite improvements in their taxonomy, some bacteroides are still misclassified. Suggestions for further improvements in the taxonomy of bacteroides are made.

摘要

拟杆菌属的定义较为宽泛,其中一些物种是口腔疾病的重要病原体,这使得与该属仅有微小相似性的分离株也被归入其中。多年来,人们进行了许多尝试来改进这一异质性细菌群体的分类学。本文综述了为此目的所使用的主要化学分类学特征和技术:色素沉着、代谢产物、全细胞脂肪酸、磷脂、类异戊二烯醌、脂多糖的碳水化合物、全细胞蛋白、肽聚糖、酶、热解质谱、DNA组成、DNA和核糖体(r)RNA的限制性片段长度多态性、DNA和RNA的同源性、DNA-rRNA杂交以及16S和5S rRNA寡核苷酸编目与测序。尽管它们的分类学有了改进,但仍有一些拟杆菌被错误分类。文中还对进一步改进拟杆菌分类学提出了建议。

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