Maruyama A, Ishihara T, Adachi N, Akaike T
Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Biomaterials. 1994 Oct;15(13):1035-42. doi: 10.1016/0142-9612(94)90088-4.
A novel method of preparing nanoparticles bearing high density carbohydrate chains on their surface is described. Carbohydrate-bearing nanoparticles of poly(lactic acid) or polystyrene were prepared by the solvent evaporation method using a carbohydrate-carrying polystyrene derivative which served as both an emulsifier and a surface coating. The diameter of the obtained nanoparticles ranged from 80 to 300 nm depending on the concentration of the polystyrene derivative. As the concentration of the polystyrene derivatives increased the nanoparticle diameter decreased, indicating that the polystyrene derivatives worked as an emulsifier. The obtained particles were specifically aggregated by carbohydrate-specific lectin, showing that the polystyrene derivative was retained on the particle surfaces and expressed carbohydrate residues. The density of carbohydrates on the particle surfaces was determined to be 3-5 molecules per square nanometre. The particles prepared by the present method were stably dispersed and hardly aggregated in aqueous media during storage and centrifugal treatment compared with the post-coated particles that were prepared by adsorbing polystyrene particles with the polystyrene derivative. In vitro study with isolated rat hepatocytes revealed that surface carbohydrate chains were recognized by hepatocytes.
描述了一种制备表面带有高密度碳水化合物链的纳米颗粒的新方法。使用既作为乳化剂又作为表面涂层的携带碳水化合物的聚苯乙烯衍生物，通过溶剂蒸发法制备了聚乳酸或聚苯乙烯的含碳水化合物纳米颗粒。所得纳米颗粒的直径根据聚苯乙烯衍生物的浓度在80至300nm范围内。随着聚苯乙烯衍生物浓度的增加，纳米颗粒直径减小，表明聚苯乙烯衍生物起到了乳化剂的作用。所得颗粒被碳水化合物特异性凝集素特异性聚集，表明聚苯乙烯衍生物保留在颗粒表面并表达碳水化合物残基。确定颗粒表面碳水化合物的密度为每平方纳米3 - 5个分子。与通过用聚苯乙烯衍生物吸附聚苯乙烯颗粒制备的后涂覆颗粒相比，通过本方法制备的颗粒在储存和离心处理期间在水性介质中稳定分散且几乎不聚集。对分离的大鼠肝细胞的体外研究表明，表面碳水化合物链被肝细胞识别。
