Chye M L, Liu C M, Tan C T
Institute of Molecular and Cell Biology, National University of Singapore.
Plant Mol Biol. 1995 Jan;27(2):419-23. doi: 10.1007/BF00020195.
A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino acid sequence of Brassica CaM with that of Arabidopsis CaM ACaM-2 and ACaM-3 showed 100% homology, which is not unusual, since both plants belong to the family Cruciferae. In situ hybridisation studies on Brassica seedlings using a digoxigenin-labelled RNA probe showed that high levels of CaM mRNA were detected in the leaf primordia and the shoot apical meristem, and to a lesser degree, in the zone of root elongation of the root tip. The occurrence of a higher rate of cell division and growth in these regions than its surrounding tissue may possibly be related to higher levels of CaM mRNA.
从芥菜中分离出了一段编码钙调蛋白（CaM）的834 bp cDNA。Northern分析表明，该cDNA与叶片、角果和花柄中约0.9 kb的mRNA杂交。基因组Southern分析表明芥菜中存在一个CaM多基因家族。芥菜CaM预测氨基酸序列与拟南芥CaM ACaM - 2和ACaM - 3的比较显示出100%的同源性，这并不罕见，因为这两种植物都属于十字花科。使用地高辛标记的RNA探针对芥菜幼苗进行原位杂交研究表明，在叶原基和茎尖分生组织中检测到高水平的CaM mRNA，而在根尖的根伸长区检测到的水平较低。这些区域比其周围组织具有更高的细胞分裂和生长速率，这可能与更高水平的CaM mRNA有关。
