Hills M J, Dann R, Lydiate D, Sharpe A
Department of Brassica and Oilseeds Research, John Innes Centre, Colney, Norwich, UK.
Plant Mol Biol. 1994 Aug;25(5):917-20. doi: 10.1007/BF00028886.
A cDNA encoding an acyl-CoA-binding protein (ACBP) homologue has been cloned from a lambda gt11 library made from mRNA isolated from developing seeds of oilseed rape (Brassica napus L.). The derived amino acid sequence reveals a protein 92 amino acids in length which is highly conserved when compared with ACBP sequences from yeast, cow, man and fruit fly. Southern blot analysis of Brassica napus genomic DNA revealed the presence of 6 genes, 3 derived from the Brassica rapa parent and 3 from Brassica oleracea. Northern blot analysis showed that ACBP genes are expressed strongly in developing embryo, flowers and cotyledons of seedlings and to a lesser extent in leaves and roots.
从一个由油菜（甘蓝型油菜L.）发育种子中分离的mRNA构建的λgt11文库中克隆出了一个编码酰基辅酶A结合蛋白（ACBP）同源物的cDNA。推导的氨基酸序列显示该蛋白长度为92个氨基酸，与来自酵母、牛、人和果蝇的ACBP序列相比具有高度保守性。甘蓝型油菜基因组DNA的Southern杂交分析表明存在6个基因，其中3个来自白菜亲本，3个来自甘蓝亲本。Northern杂交分析表明ACBP基因在发育中的胚、花和幼苗子叶中强烈表达，在叶和根中表达较弱。
