Cameron N E, Cotter M A
Department of Biomedical Sciences, University of Aberdeen, Marischal College, UK.
Acta Diabetol. 1994 Dec;31(4):220-5. doi: 10.1007/BF00571955.
Evening primrose oil (EPO) is rich in the omega-6 essential fatty acid component, gamma-linolenic acid. The aim of the investigation was to determine whether EPO treatment prevented a reduction in sciatic nerve perfusion and oxygenation in streptozotocin-diabetic rats. Rats were treated from diabetes induction with 10 g EPO kg-1 day-1. Sciatic blood flow was measured by microelectrode polarography and hydrogen clearance. Diabetes caused 47.7% +/- 3.4% (P < 0.001) and 58.8% +/- 4.8% (P < 0.001) reduction in the nutritive (capillary) and the non-nutritive (large vessel) components of endoneurial blood flow, respectively, which were prevented by EPO. Treatment had no significant effect on nutritive flow in non-diabetic rats; however, the rate of non-nutritive flow increased by 97.7% +/- 38.9% (P < 0.01). Sciatic endoneurial oxygen tension was measured by microelectrode polarography. Diabetes resulted in a 44.7% +/- 3.4% reduction in mean oxygen tension (P < 0.001), which was largely (82.3% +/- 10.2%) prevented by EPO treatment (P < 0.001). Thus, EPO prevents impairment of blood flow and endoneurial oxygenation in experimental diabetes. It is likely that this neurovascular action accounts for the beneficial effects of treatment on nerve function in diabetic rats and patients.
月见草油（EPO）富含ω-6必需脂肪酸成分γ-亚麻酸。本研究的目的是确定EPO治疗是否能预防链脲佐菌素诱导的糖尿病大鼠坐骨神经灌注和氧合的降低。大鼠自诱导糖尿病起接受10 g EPO kg-1 day-1的治疗。通过微电极极谱法和氢清除法测量坐骨神经血流量。糖尿病分别导致神经内膜血流的营养性（毛细血管）和非营养性（大血管）成分减少47.7%±3.4%（P<0.001）和58.8%±4.8%（P<0.001），而EPO可预防这种减少。该治疗对非糖尿病大鼠的营养性血流无显著影响；然而，非营养性血流速率增加了97.7%±38.9%（P<0.01）。通过微电极极谱法测量坐骨神经内膜氧张力。糖尿病导致平均氧张力降低44.7%±3.4%（P<0.001），而EPO治疗可在很大程度上（82.3%±10.2%）预防这种降低（P<0.001）。因此，EPO可预防实验性糖尿病中血流和神经内膜氧合的损害。这种神经血管作用可能解释了该治疗对糖尿病大鼠和患者神经功能的有益影响。
