Niese D, Grosse-Wilde H, Dupont B, Du Toit E, Johannsen R, Suciu-Foca N, Stroehmann I, Rittner C
Hum Genet. 1978 Jul 12;43(1):23-30. doi: 10.1007/BF00396474.
This study was undertaken to get more insight into the previously suggested heterogeneity of the HLA--DW3 cluster. Preliminary evidence of DW3 heterogeneity was derived from results of intrafamilial mixed lymphocyte culture tests (MLC) where cells of apparently homozygous offspring revealed unexpected stimulations of one of the parents' cells. Therefore, 15 different homozygous typing cells (HTCs) of DW3 specificity were tested against 43 HLA-DW3 heterozygous individuals. The response patterns of the 43 HLA-DW3 heterozygous cells toward 13 HTCs leads to the definition of at least three groups of DW3 stimulating cells. According to these patterns, four groups of responding cells could be classified. These results were confirmed by a MLC checkerboard experiment running all DW3-HTCs against each other. Discussing all possible explanations for these observations, the authors conclude that the existence of DW3 subtypes having some properties in common is the most likely interpretation of the results obtained. Family segregation studies will be needed to define the genotypic situation of the DW3 cluster.
本研究旨在更深入地了解先前提出的HLA - DW3簇的异质性。DW3异质性的初步证据来自家族内混合淋巴细胞培养试验（MLC）的结果，在该试验中，明显纯合子后代的细胞对父母一方的细胞产生了意外的刺激。因此，对15种不同的DW3特异性纯合分型细胞（HTC）进行了检测，检测对象为43名HLA - DW3杂合个体。43个HLA - DW3杂合细胞对13个HTC的反应模式导致定义了至少三组DW3刺激细胞。根据这些模式，可以将四组反应细胞进行分类。通过让所有DW3 - HTC相互进行MLC棋盘试验，证实了这些结果。在讨论对这些观察结果的所有可能解释时，作者得出结论，具有某些共同特性的DW3亚型的存在是对所获结果最可能的解释。需要进行家系分离研究来确定DW3簇的基因型情况。
