Pérez-Payá E, Houghten R A, Blondelle S E
Torrey Pines Institute for Molecular Studies, San Diego, CA.
Pept Res. 1994 Nov-Dec;7(6):286-8.
Peptides able to adopt an environment-dependent secondary structure represent attractive models for enzyme mimics. An approach to design new synthetic enzyme-like peptides involves the use of binding-step based mimics. The polypeptide melittin, based on its intrinsic ability to bind to phospholipids, was selected as a potential binding-step based catalytic mimic. Synthetic melittin was found to exhibit weak but reproducible phospholipase-like activity. The rate enhancement of the hydrolysis of phospholipids, although far from that of the natural enzyme, was encouraging as a first step in the development of new catalytic peptides.
能够形成环境依赖性二级结构的肽是酶模拟物的有吸引力的模型。设计新型合成酶样肽的一种方法涉及使用基于结合步骤的模拟物。基于其与磷脂结合的固有能力，多肽蜂毒素被选为基于结合步骤的潜在催化模拟物。发现合成蜂毒素表现出微弱但可重复的磷脂酶样活性。磷脂水解的速率增强虽然远低于天然酶，但作为开发新型催化肽的第一步是令人鼓舞的。
