采用电子捕获气相色谱法测定人血浆和尿液中的水合氯醛及其代谢物（三氯乙醇和三氯乙酸）。

Determination of chloral hydrate and its metabolites (trichloroethanol and trichloracetic acid) in human plasma and urine using electron capture gas chromatography.

作者信息

Humbert L, Jacquemont M C, Leroy E, Leclerc F, Houdret N, Lhermitte M

机构信息

Laboratoire de Biochimie, Hôpital Calmette, Lille, France.

出版信息

Biomed Chromatogr. 1994 Nov-Dec;8(6):273-7. doi: 10.1002/bmc.1130080604.

DOI:10.1002/bmc.1130080604

PMID:7888728

Abstract

Capillary gas chromatography with electron capture detection is described for the quantification of chloral hydrate (CH) and is metabolites trichloroethanol (TCE) and trichloroacetic acid (TCA) in 0.1-1 mL of plasma samples. The method, with 2,2'-dichloroethanol (DCE) as internal standard, involved extraction of chloral hydrate and trichloroethanol with diethyl ether and methylation of trichloroacetic acid with 3-methyl-1-tolyltriazene (MTT), followed by diethyl ether extraction. The method has a detection limit of 5 ng/mL for CH and TCE and 10 ng/mL for TCA and also allows the determination of TCE-glucuronide in 0.1-1 mL of plasma samples. It exhibits good linearity and precision. The method was applied to samples of plasma from a neonate after a single dose of 40 mg/kg of chloral hydrate and from an adult after a single dose of 6.25 mg/kg.

摘要

本文描述了一种采用电子捕获检测的毛细管气相色谱法，用于定量测定0.1 - 1 mL血浆样品中的水合氯醛（CH）及其代谢物三氯乙醇（TCE）和三氯乙酸（TCA）。该方法以2,2'-二氯乙醇（DCE）作为内标，先用乙醚萃取水合氯醛和三氯乙醇，再用3 - 甲基 - 1 - 甲苯基三氮烯（MTT）对三氯乙酸进行甲基化，随后用乙醚萃取。该方法对CH和TCE的检测限为5 ng/mL，对TCA的检测限为10 ng/mL，还能测定0.1 - 1 mL血浆样品中的TCE - 葡萄糖醛酸苷。该方法具有良好的线性和精密度。该方法应用于一名新生儿单次服用40 mg/kg水合氯醛后的血浆样品以及一名成年人单次服用6.25 mg/kg后的血浆样品。