采用气相色谱/质谱联用技术对动态顶空法进行评估，以测定生物样品中的1,1,1-三氯乙烷、三氯乙醇和三氯乙酸。

Evaluation of dynamic headspace with gas chromatography/mass spectrometry for the determination of 1,1,1-trichloroethane, trichloroethanol, and trichloroacetic acid in biological samples.

作者信息

Johns Douglas O, Dills Russell L, Morgan Michael S

机构信息

Department of Environmental and Occupational Health Sciences, University of Washington, Box 357234, Seattle, WA 98195-7234, USA.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Mar 25;817(2):255-61. doi: 10.1016/j.jchromb.2004.12.013.

DOI:10.1016/j.jchromb.2004.12.013

PMID:15686993

Abstract

A sensitive and reproducible method is described for the analysis of trichloroacetic acid in urine and 1,1,1-trichloroethane in blood using dynamic headspace GC/MS. Samples were analyzed using the soil module of a modified purge and trap autosampler to facilitate the use of disposable purging vessels. Coefficients of variation were below 3.5% for both analytes, and response was linear in the range of 0.01-7.0 microg/ml for trichloroacetic acid and 0.9 ng/ml-2.2 microg/ml for 1,1,1-trichloroethane. Attempts at using dynamic headspace for the analysis of trichloroethanol in urine were unsuccessful.

摘要

描述了一种灵敏且可重复的方法，用于使用动态顶空气相色谱/质谱法分析尿液中的三氯乙酸和血液中的1,1,1-三氯乙烷。使用改良的吹扫捕集自动进样器的土壤模块对样品进行分析，以方便使用一次性吹扫容器。两种分析物的变异系数均低于3.5%，三氯乙酸在0.01 - 7.0微克/毫升范围内、1,1,1-三氯乙烷在0.9纳克/毫升 - 2.2微克/毫升范围内响应呈线性。尝试使用动态顶空分析法分析尿液中的三氯乙醇未成功。