Baba Y, Tomisaki R, Sumita C, Tsuhako M, Miki T, Ogihara T
Department of Chemistry, Kobe Pharmaceutical University, Japan.
Biomed Chromatogr. 1994 Nov-Dec;8(6):291-3. doi: 10.1002/bmc.1130080608.
High-resolution separation of a PCR product from a mixture of DNA restriction fragments was achieved using capillary gel electrophoresis. The capillary gel electrophoretic separation gives an excellent resolution of two fragments of the 500-bp PCR product and the 506-bp DNA fragment, which differs by only 6 bp, and the complete separation of a broader chain length range of DNA afragments up to 12 kbp within 20 min. The plate number of gel-filled capillary was achieved to be 2 million plates per meter. Capillary gel electrophoresis is applied to the gene diagnosis for heart disease through apolipoprotein E genotyping. The advantages and limitations of capillary gel electrophoresis in the application to PCR analysis and DNA diagnosis are discussed.
使用毛细管凝胶电泳实现了从DNA限制性片段混合物中对PCR产物的高分辨率分离。毛细管凝胶电泳分离能出色地分辨出500 bp的PCR产物片段和506 bp的DNA片段这两个仅相差6 bp的片段，并且能在20分钟内完全分离出链长范围更广的高达12 kbp的DNA片段。填充凝胶的毛细管的塔板数达到了每米200万块塔板。毛细管凝胶电泳通过载脂蛋白E基因分型应用于心脏病的基因诊断。讨论了毛细管凝胶电泳在应用于PCR分析和DNA诊断中的优点和局限性。
