Arakawa H, Uetanaka K, Maeda M, Tsuji A
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
J Chromatogr A. 1994 Mar 25;664(1):89-98. doi: 10.1016/0021-9673(94)80632-2.
Capillary gel electrophoresis (CGE) was studied for the direct analysis of polymerase chain reaction (PCR) amplified samples. A low cross-linked polyacrylamide gel (3%T, 0.5%C) was used for CGE with treated and untreated silica capillaries. CGE showed high reproducibility and resolution in the separation of DNA fragments (ca. 100-1000 base pairs) produced by PCR. The CGE system was applied to the detection of an amplification refractory mutation system (ARMS) and PCR-restriction fragment length polymorphism (PCR-RFLP), which are detection methods of single base substitution in genes using PCR. With the CGE system, full automation of PCR product detection is feasible.
研究了毛细管凝胶电泳（CGE）用于直接分析聚合酶链反应（PCR）扩增样品。采用低交联聚丙烯酰胺凝胶（3%T，0.5%C），在经过处理和未处理的硅胶毛细管上进行CGE。CGE在分离由PCR产生的DNA片段（约100 - 1000个碱基对）时显示出高重现性和分辨率。该CGE系统应用于扩增不应性突变系统（ARMS）和PCR - 限制性片段长度多态性（PCR - RFLP）的检测，这两种方法是利用PCR检测基因中单碱基取代的方法。使用CGE系统，PCR产物检测的完全自动化是可行的。
